恶性疟原虫苹果酸脱氢酶的结晶和初步 X 射线衍射。

Carsten Wrenger, Ingrid B Müller, Sabine Butzloff, Rositsa Jordanova, Sergey Lunev, Matthew R Groves
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引用次数: 0

摘要

报告了恶性疟原虫中的苹果酸脱氢酶(MDH)(PfMDH)的表达、纯化、结晶和初步 X 射线衍射表征。为了深入了解 PfMDH 的功能和作用,对该蛋白进行了纯化。纯化后的蛋白质在空间群 P1 中结晶,单位晶胞参数为 a = 72、b = 157、c = 159 Å、α = 105、β = 101、γ = 95°。所得晶体的最大衍射分辨率为 2.24 Å,该结构已通过分子置换得到解决,不对称单元中有 16 个单体。这 16 个单体排列成四个独立的四聚体,这与之前证明 PfMDH 存在四聚体溶液态的报道一致。PfMDH的X射线结构有望阐明PfMDH与其他MDH家族成员在催化方面的差异,并为基于结构设计特异性PfMDH抑制剂和一般MDH抑制剂提供依据。
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Crystallization and preliminary X-ray diffraction of malate dehydrogenase from Plasmodium falciparum.

The expression, purification, crystallization and preliminary X-ray diffraction characterization of malate dehydrogenase (MDH) from the malarial parasite Plasmodium falciparum (PfMDH) are reported. In order to gain a deeper understanding of the function and role of PfMDH, the protein was purified to homogeneity. The purified protein crystallized in space group P1, with unit-cell parameters a = 72, b = 157, c = 159 Å, α = 105, β = 101, γ = 95°. The resulting crystals diffracted to a maximal resolution of 2.24 Å and the structure has been solved by molecular replacement, with 16 monomers in the asymmetric unit. The 16 monomers are arranged into four independent tetramers, in agreement with previous reports demonstrating the tetrameric solution state of PfMDH. The X-ray structure of PfMDH is expected to clarify the differences in catalysis by PfMDH compared with other MDH family members and to provide a basis for the structure-based design of specific PfMDH inhibitors as well as general MDH inhibitors.

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期刊介绍: Acta Crystallographica Section F is a rapid structural biology communications journal. Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal. The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles. Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.
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