高血糖可引起糖尿病大鼠海马和下丘脑11β-羟基类固醇脱氢酶1型和过氧化物酶体增殖物激活受体-γ表达的变化。

Experimental Diabetes Research Pub Date : 2012-01-01 Epub Date: 2012-06-26 DOI:10.1155/2012/107130
Wen-wen Qi, Li-yong Zhong, Xiao-rong Li, Guang Li, Zhao-xia Liu, Jin-feng Hu, Nai-hong Chen
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引用次数: 18

摘要

本文首先采用免疫组化方法观察恒定高血糖组、对照组和波动血糖组海马和下丘脑11β-HSD1和PPAR-γ的表达差异。然而,无论是11β-HSD1还是PPAR-γ的表达,在对照组和波动血糖组之间均无统计学差异。因此,我们去掉波动血糖组,只保留恒定高血糖组和对照组,饲喂8周。诱导8周后,Western Blot结果显示,与对照组相比,持续高血糖组海马和下丘脑11β-HSD1表达升高,PPAR-γ表达降低。持续高血糖组8周后MORRIS水迷宫认知功能受损,血清皮质酮水平降低,血清ACTH浓度升高。我们推测认知功能障碍可能与中枢神经系统11β-HSD1和PPAR-γ的异常表达有关。11β-HSD1是一种调节酶,将失活的11-脱氢皮质酮转化为活性的糖皮质激素皮质酮,从而放大局部组织的GC作用。众所周知,局部GC水平高会影响认知功能。此外,ppar是一种与认知有关的保护性受体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Hyperglycemia induces the variations of 11β-hydroxysteroid dehydrogenase type 1 and peroxisome proliferator-activated receptor-γ expression in hippocampus and hypothalamus of diabetic rats.

In this paper, we first observed that there were differences in expressions of 11β-HSD1 and PPAR-γ, in hippocampi and hypothalami, among constant hyperglycemia group, control group and the fluctuant glycemia group, using Immunohistochemical analysis. However, whether in expression o f 11β-HSD1 or PPAR-γ, there were no statistic differences between the control group or the fluctuant glycemia group. So, we removed the fluctuant glycemia group, retaining only constant hyperglycemia group and control group, being fed for 8 weeks. After 8 weeks of induction, 11β-HSD1 expression increased and PPAR-γ expression decreased in the constant hyperglycemia group compared with control group, both in hippocampi and hypothalami, by Western Blot. The constant hyperglycemia group also showed impaired cognition in MORRIS watermaze, lower serum corticosterone level, and higher Serum ACTH concentration after 8 weeks. We inferred that the cognition impairment may be related to the abnormal expression of 11β-HSD1 and PPAR-γ in central nerves system. As for 11β-HSD1 is a regulating enzyme, converting the inactive 11-dehydrocorticosterone into the active glucocorticoid corticosterone, thus amplifying GC action in local tissues. It is also well known that high local GC levels can affect the cognitive function. In addition, PPAR-a protective receptor, which is related to cognition.

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Experimental Diabetes Research
Experimental Diabetes Research 医学-内分泌学与代谢
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