Karin Reuter, Stefan Schlatt, Jens Ehmcke, Joachim Wistuba
{"title":"事实还是虚构:体外精子发生。","authors":"Karin Reuter, Stefan Schlatt, Jens Ehmcke, Joachim Wistuba","doi":"10.4161/spmg.21983","DOIUrl":null,"url":null,"abstract":"<p><p>Many previous studies have aimed at spermatogenesis of male murine germ cells in vitro, but no efficient system has been established yet that covers the entire process of mammalian spermatogenesis in a culture dish permanently. In this review, we report on the requirements of spermatogenesis and the current state of different culture methods using testicular tissue fragments, single cell suspensions or three-dimensional culture environments.</p>","PeriodicalId":22074,"journal":{"name":"Spermatogenesis","volume":"2 4","pages":"245-252"},"PeriodicalIF":0.0000,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/spmg.21983","citationCount":"14","resultStr":"{\"title\":\"Fact or fiction: In vitro spermatogenesis.\",\"authors\":\"Karin Reuter, Stefan Schlatt, Jens Ehmcke, Joachim Wistuba\",\"doi\":\"10.4161/spmg.21983\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Many previous studies have aimed at spermatogenesis of male murine germ cells in vitro, but no efficient system has been established yet that covers the entire process of mammalian spermatogenesis in a culture dish permanently. In this review, we report on the requirements of spermatogenesis and the current state of different culture methods using testicular tissue fragments, single cell suspensions or three-dimensional culture environments.</p>\",\"PeriodicalId\":22074,\"journal\":{\"name\":\"Spermatogenesis\",\"volume\":\"2 4\",\"pages\":\"245-252\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.4161/spmg.21983\",\"citationCount\":\"14\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Spermatogenesis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4161/spmg.21983\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spermatogenesis","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4161/spmg.21983","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Many previous studies have aimed at spermatogenesis of male murine germ cells in vitro, but no efficient system has been established yet that covers the entire process of mammalian spermatogenesis in a culture dish permanently. In this review, we report on the requirements of spermatogenesis and the current state of different culture methods using testicular tissue fragments, single cell suspensions or three-dimensional culture environments.
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