聚乳酸-羟基乙酸核+聚n -异丙基丙烯酰胺壳纳米粒子体系的合成与表征。

Biomatter Pub Date : 2012-10-01 DOI:10.4161/biom.22494
Aaron M Kosinski, Jamie L Brugnano, Brandon L Seal, Frances C Knight, Alyssa Panitch
{"title":"聚乳酸-羟基乙酸核+聚n -异丙基丙烯酰胺壳纳米粒子体系的合成与表征。","authors":"Aaron M Kosinski,&nbsp;Jamie L Brugnano,&nbsp;Brandon L Seal,&nbsp;Frances C Knight,&nbsp;Alyssa Panitch","doi":"10.4161/biom.22494","DOIUrl":null,"url":null,"abstract":"<p><p>Poly(lactic-co-glycolic acid) (PLGA) is a popular material used to prepare nanoparticles for drug delivery. However, PLGA nanoparticles lack desirable attributes including active targeting abilities, resistance to aggregation during lyophilization, and the ability to respond to dynamic environmental stimuli. To overcome these issues, we fabricated a nanoparticle consisting of a PLGA core encapsulated within a shell of poly(N-isopropylacrylamide). Dynamic light scattering and transmission electron microscope imaging were used to characterize the nanoparticles, while an MTT assay and ELISA suggested biocompatibility in THP1 cells. Finally, a collagen type II binding assay showed successful modification of these nanoparticles with an active targeting moiety.</p>","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"2 4","pages":"195-201"},"PeriodicalIF":0.0000,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/biom.22494","citationCount":"13","resultStr":"{\"title\":\"Synthesis and characterization of a poly(lactic-co-glycolic acid) core + poly(N-isopropylacrylamide) shell nanoparticle system.\",\"authors\":\"Aaron M Kosinski,&nbsp;Jamie L Brugnano,&nbsp;Brandon L Seal,&nbsp;Frances C Knight,&nbsp;Alyssa Panitch\",\"doi\":\"10.4161/biom.22494\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Poly(lactic-co-glycolic acid) (PLGA) is a popular material used to prepare nanoparticles for drug delivery. However, PLGA nanoparticles lack desirable attributes including active targeting abilities, resistance to aggregation during lyophilization, and the ability to respond to dynamic environmental stimuli. To overcome these issues, we fabricated a nanoparticle consisting of a PLGA core encapsulated within a shell of poly(N-isopropylacrylamide). Dynamic light scattering and transmission electron microscope imaging were used to characterize the nanoparticles, while an MTT assay and ELISA suggested biocompatibility in THP1 cells. Finally, a collagen type II binding assay showed successful modification of these nanoparticles with an active targeting moiety.</p>\",\"PeriodicalId\":8891,\"journal\":{\"name\":\"Biomatter\",\"volume\":\"2 4\",\"pages\":\"195-201\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.4161/biom.22494\",\"citationCount\":\"13\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomatter\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4161/biom.22494\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomatter","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4161/biom.22494","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 13

摘要

聚乳酸-羟基乙酸(PLGA)是制备纳米药物递送材料的常用材料。然而,PLGA纳米颗粒缺乏理想的特性,包括主动靶向能力,在冻干过程中抵抗聚集,以及对动态环境刺激的响应能力。为了克服这些问题,我们制造了一种由聚n -异丙基丙烯酰胺外壳内包裹的PLGA核心组成的纳米颗粒。采用动态光散射和透射电镜成像对纳米颗粒进行了表征,MTT试验和ELISA检测表明纳米颗粒在THP1细胞中的生物相容性。最后,胶原II型结合试验显示,这些纳米颗粒被成功地修饰为具有活性靶向部分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

摘要图片

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Synthesis and characterization of a poly(lactic-co-glycolic acid) core + poly(N-isopropylacrylamide) shell nanoparticle system.

Poly(lactic-co-glycolic acid) (PLGA) is a popular material used to prepare nanoparticles for drug delivery. However, PLGA nanoparticles lack desirable attributes including active targeting abilities, resistance to aggregation during lyophilization, and the ability to respond to dynamic environmental stimuli. To overcome these issues, we fabricated a nanoparticle consisting of a PLGA core encapsulated within a shell of poly(N-isopropylacrylamide). Dynamic light scattering and transmission electron microscope imaging were used to characterize the nanoparticles, while an MTT assay and ELISA suggested biocompatibility in THP1 cells. Finally, a collagen type II binding assay showed successful modification of these nanoparticles with an active targeting moiety.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Effect of grain sizes on mechanical properties and biodegradation behavior of pure iron for cardiovascular stent application. Fabrication and surface modification of poly lactic acid (PLA) scaffolds with epidermal growth factor for neural tissue engineering. The effect of oligo(trimethylene carbonate) addition on the stiffness of acrylic bone cement Enhanced bioactivity of glass ionomer cement by incorporating calcium silicates Development of a novel carrier optimized for cell sheet transplantation.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1