妊娠晚期和哺乳期早期饲粮脂质对奶牛肝脏代谢和炎症基因网络表达的影响——以ppar为重点

Gene regulation and systems biology Pub Date : 2013-06-11 Print Date: 2013-01-01 DOI:10.4137/GRSB.S12005
Haji Akbar, Eduardo Schmitt, Michael A Ballou, Marcio N Corrêa, Edward J Depeters, Juan J Loor
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引用次数: 25

摘要

多不饱和(PUFA)长链脂肪酸(LCFAs)比饱和LCFA更有效地引起单核苷酸多态性的分子和组织功能变化。相对于分娩-21 ~ 10 d,奶牛分别饲喂不添加LCFA(对照)、饱和LCFA (SFAT;主要是16:0和18:0),或鱼油(fish;high-PUFA)。在第-14天和第10天用定量RT-PCR法检测27个基因。核受体共激活因子(CARM1, MED1), LCFA代谢(ACSL1, SCD, ACOX1)和炎症(IL6, TBK1, IKBKE)基因的表达在-14天SFAT低于对照组。然而,在-14天和10天,FISH组SCD的表达明显低于对照组或SFAT组。在第10天,FISH导致LCFA代谢(CD36、PLIN2、ACSL1、ACOX1)、细胞内能量(UCP2、STK11、PRKAA1)、新生胆固醇合成(SREBF2)、炎症(IL6、TBK1、IKBKE)和核受体信号基因(PPARD、MED1、NRIP1)的表达进一步降低。PPARA和RXRA的表达未见变化。饲喂SFAT的奶牛第10天DGAT2、PLIN2、ACSL1和ACOX1较-14天增加,表明β -氧化和脂滴(LD)形成均上调。然而,不同治疗组的肝脏甘油三酯浓度相似。肝因子FGF21和糖异生基因PC和PCK1在第10天明显高于对照组。在补充水平下,饲喂饱和脂肪的奶牛分娩后代谢基因谱的变化表明,它们对脂肪酸的吸收和细胞内处理能力更强,而不会过度储存LD。
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Dietary Lipid During Late-Pregnancy and Early-Lactation to Manipulate Metabolic and Inflammatory Gene Network Expression in Dairy Cattle Liver with a Focus on PPARs.

Polyunsaturated (PUFA) long-chain fatty acids (LCFAs) are more potent in eliciting molecular and tissue functional changes in monogastrics than saturated LCFA. From -21 through 10 days relative to parturition dairy cows were fed no supplemental LCFA (control), saturated LCFA (SFAT; mainly 16:0 and 18:0), or fish oil (FISH; high-PUFA). Twenty-seven genes were measured via quantitative RT-PCR in liver tissue on day -14 and day 10. Expression of nuclear receptor co-activators (CARM1, MED1), LCFA metabolism (ACSL1, SCD, ACOX1), and inflammation (IL6, TBK1, IKBKE) genes was lower with SFAT than control on day -14. Expression of SCD, however, was markedly lower with FISH than control or SFAT on both -14 and 10 days. FISH led to further decreases in expression on day 10 of LCFA metabolism (CD36, PLIN2, ACSL1, ACOX1), intracellular energy (UCP2, STK11, PRKAA1), de novo cholesterol synthesis (SREBF2), inflammation (IL6, TBK1, IKBKE), and nuclear receptor signaling genes (PPARD, MED1, NRIP1). No change in expression was observed for PPARA and RXRA. The increase of DGAT2, PLIN2, ACSL1, and ACOX1 on day 10 versus -14 in cows fed SFAT suggested upregulation of both beta-oxidation and lipid droplet (LD) formation. However, liver triacylglycerol concentration was similar among treatments. The hepatokine FGF21 and the gluconeogenic genes PC and PCK1 increased markedly on day 10 versus -14 only in controls. At the levels supplemented, the change in the profile of metabolic genes after parturition in cows fed saturated fat suggested a greater capacity for uptake of fatty acids and intracellular handling without excessive storage of LD.

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