总状菊水提物的抗诱变和抗细胞凋亡作用。f. 4- nqo诱导的小鼠遗传损伤。

ISRN Pharmacology Pub Date : 2013-09-03 eCollection Date: 2013-01-01 DOI:10.1155/2013/768359
P Arumugam, M Murugan
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引用次数: 12

摘要

本研究是通过实验研究总状菊根提取物对4-硝基喹啉-1-氧化物(4-NQO)诱导的小鼠骨髓细胞DNA损伤和凋亡的保护作用,来验证其在印度作为传统药物使用的一部分。将总状菊水根提取物(100、200和400 mg/kg bw)(含4-NQO和不含4-NQO)与对照物(H2O)连续口服5 d。第6天腹腔注射4-NQO (7.5 mg/kg bw)。24h后处死动物,提取骨髓细胞进行微核和凋亡分析。采用Annexin V-FITC检测试剂盒检测ARE (400mg /kg bw)的抗凋亡作用。4-NQO产生微核多染红细胞(mnpce)的频率约为对照值的4.7倍,为14.29 mnpce /2500 pce。与剂量相比,ARE预处理显著降低了mnpce频率(39-72%),并且与单独使用4-NQO相比,pce / nce比值增加。4- nqo诱导的凋亡细胞总数约为对照组的12%,经400 mg/kg bw ARE预处理后,4- nqo诱导的凋亡细胞总数显著降低(P < 0.05)至3.5%。本研究首次报道了总状花对4- nqo诱导的小鼠骨髓细胞DNA损伤和凋亡的保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Antimutagenic and Antiapoptotic Effects of Aqueous Root Extract of Inula racemosa Hook. f. on 4-NQO-Induced Genetic Damage in Mice.

The present study was performed as part of an attempt to authenticate the use of Inula racemosa root extract as traditional medicine in India by experimentally investigating their protective effects on 4-nitroquinoline-1-oxide (4-NQO) induced DNA damage and apoptosis in mice bone marrow cells. Aqueous root extract (ARE) of Inula racemosa (100, 200 and 400 mg/kg bw) with and without 4-NQO along with vehicle control (H2O) were administered orally for five consecutive days. 4-NQO (7.5 mg/kg bw) was injected intraperitoneally to the mice on the sixth day. After 24 h, the animals were sacrificed and extracted bone marrow cells were used for micronuclei and apoptotic analysis. Antiapoptotic effect of ARE (400 mg/kg bw) was measured by the use of Annexin V-FITC assay kit. 4-NQO generated the frequency of micronucleated polychromatic erythrocytes (MnPCEs) by about 4.7 times the control value, 14.29 MnPCEs/2500 PCEs. Pretreatment with ARE significantly reduced the MnPCEs frequency (39-72%) with respect to their doses, and increased PCEs/NCEs ratio was observed over the 4-NQO alone. 4-NQO-induced total apoptotic cells were about 12% over the control which was significantly (P < 0.05) brought down to 3.5% by pretreatment with 400 mg/kg bw of ARE. This was the first report that recorded the protective effects of I. racemosa on 4-NQO-induced DNA damage and apoptosis in mice bone marrow cells.

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