番茄花叶病毒抗性蛋白 Tm-1 的抑制结构域的结晶和初步 X 射线晶体学分析。

Masahiko Kato, Yuichiro Kezuka, Chihoko Kobayashi, Kazuhiro Ishibashi, Takamasa Nonaka, Masayuki Ishikawa, Estuko Katoh
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引用次数: 0

摘要

Tm-1是番茄花叶病毒RNA复制的抑制蛋白,含有两个保守结构域:N-端为未定性结构域,C-端为TIM-桶状结构域。Tm-1 的 N 端结构域具有抑制活性,但其三维结构尚未确定。本文报告了 Tm-1 N 端结构域的结晶和初步 X 射线衍射。从硒代蛋氨酸标记的晶体中收集了三波长 MAD 数据集,并将其处理为 2.7 Å 分辨率。该晶体属于三菱空间群 P1,单位晶胞参数为 a = 77.97、b = 105.28、c = 110.62 Å、α = 94.6、β = 109.3、γ = 108.0°。
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Crystallization and preliminary X-ray crystallographic analysis of the inhibitory domain of the tomato mosaic virus resistance protein Tm-1.

Tm-1, an inhibitor protein of Tomato mosaic virus RNA replication, contains two conserved domains: an uncharacterized domain at its N-terminus and a TIM-barrel-like domain at its C-terminus. The N-terminal domain of Tm-1 has an inhibitory activity and its three-dimensional structure has not been determined. Here, the crystallization and preliminary X-ray diffraction of the N-terminal domain of Tm-1 are reported. A three-wavelength MAD data set was collected from a selenomethionine-labelled crystal and processed to 2.7 Å resolution. The crystal belonged to the triclinic space group P1, with unit-cell parameters a = 77.97, b = 105.28, c = 110.62 Å, α = 94.6, β = 109.3, γ = 108.0°.

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期刊介绍: Acta Crystallographica Section F is a rapid structural biology communications journal. Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal. The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles. Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.
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