Novel hybrid membrane of chitosan/poly (ε-caprolactone) for tissue engineering.

We investigated the potential use of 3D hybrid membrane: poly (ε-caprolactone) (PCL) mesh using rotary jet spinning with subsequent chitosan (CH) coating. The morphological examinations by scanning electron microscopy (SEM) were proved the efficiency of this technique on obtaining relative homogeneous PCL fiber mats (15,49±4,1µm), with high surface porosity (1,06±0,41µm) and effective CH coating. The feasibility of rotary jet spinning allowed the solvent evaporation during the process; this fact was verified by differential scanning calorimetry (DSC), indeed also had verified changes in thermal properties on the hybrid membrane, since the present of CH. It was investigated the mechanical properties of the hybrid membrane and CH film, the data were that the samples presents good tensile modulus but low strain at the break. In addition, it was verified the biocompatibility properties in vitro using Vero cells. PCL mesh demonstrated cells more spread vastly in the pore surface, with attachments in between fibers indicating the potential for cell adhesion. The films samples (CH and hybrid membrane) resulted in a cells layer on the surfaces with an intense staining (metachromasy), which is the result of cells more active. The cell counting -5 days of culture- and the MTT assay -21 days of culture- demonstrated that the materials tested proved to be different from the positive control and equal to each other and this fact, in our view, this indicates a satisfactory proliferation. Thus, based on the results here, this novel hybrid membrane provides an attractive material for tissue engineering applications.