煌胫毒蛙 Allobates femoralis（石斛科）七个新的多态性微卫星位点的特征及其在其他三个石斛物种中的跨物种实用性。

IF 1.1 4区生物学 Q3 ZOOLOGY Herpetological Journal Pub Date : 2013-07-01

Eva Ringler, Andrius Pašukonis, Walter Hödl, Max Ringler

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引用次数: 0

摘要

在这里，我们记录了针对煌髀毒蛙（Allobates femoralis，Dendrobatidae）开发的七个新型多态性微卫星标记。我们在法属圭亚那 "Saut Pararé "野外地点的 100 个个体（50 雄 50 雌）中发现每个位点有 6 至 27 个等位基因，平均杂合度为 0.79。其中一个基因座（Afem23）明显偏离了哈代-温伯格平衡。我们没有发现任何证据表明新位点之间存在联系不平衡，也没有发现新位点与七个已经描述过的大花杓鹬标记物之间存在联系不平衡。我们还报告了其他三个石斛物种（A. talamancae、Dendrobates tinctorius 和 Oophaga pumilio）中一些标记的跨物种扩增情况。

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Characterization of seven new polymorphic microsatellite loci in the brilliant-thighed poison frog Allobates femoralis (Dendrobatidae), and their cross-species utility in three other dendrobatid species.

Here we document the development of seven novel polymorphic microsatellite markers for the brilliant-thighed poison frog Allobates femoralis (Dendrobatidae). We found between six and 27 alleles per locus in 100 individuals (50 males, 50 females) from the field site 'Saut Pararé', French Guiana, with an average observed heterozygosity of 0.79. One locus (Afem23) deviated significantly from Hardy-Weinberg equilibrium. We did not find any evidence for linkage disequilibrium among the new loci, or to seven of the already described markers for A. femoralis. We also report cross-species amplification of some of the markers in three other dendrobatid species (A. talamancae, Dendrobates tinctorius and Oophaga pumilio).

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