Ryan M Williams, Amanda R Kulick, Srilakshmi Yedlapalli, Louisa Battistella, Cyrus J Hajiran, Letha J Sooter
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引用次数: 0
摘要
溴嘧啶是一种广泛使用的除草剂，已知会污染环境系统。由于它带来的危害和低效的检测方法，有必要创建一种快速高效的传感装置。为此，我们采用了一种严格的体外选择方法，以确定对溴嘧啶具有特异性的单链 DNA 分子识别元件（MRE）。与阴性选择靶标和其他农药相比，我们发现了一种对溴嘧啶具有高亲和力（K d = 9.6 nM）和特异性的 MRE。选中的 ssDNA MRE 将作为可在野外部署的溴嘧啶检测装置中的传感元件。
In vitro selection of a single-stranded DNA molecular recognition element specific for bromacil.
Bromacil is a widely used herbicide that is known to contaminate environmental systems. Due to the hazards it presents and inefficient detection methods, it is necessary to create a rapid and efficient sensing device. Towards this end, we have utilized a stringent in vitro selection method to identify single-stranded DNA molecular recognition elements (MRE) specific for bromacil. We have identified one MRE with high affinity (K d = 9.6 nM) and specificity for bromacil compared to negative targets of selection and other pesticides. The selected ssDNA MRE will be useful as the sensing element in a field-deployable bromacil detection device.
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