Tec1在白色念珠菌性生物膜的发育、结构和完整性中的作用。

Eukaryotic Cell Pub Date : 2015-03-01 Epub Date: 2015-01-02 DOI:10.1128/EC.00224-14
Karla J Daniels, Thyagarajan Srikantha, Claude Pujol, Yang-Nim Park, David R Soll
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引用次数: 19

摘要

mtl纯合子(A: / A:或α/α)白细胞形成复杂的性生物膜,其结构与mtl杂合子(A: /α)致病生物膜相同。但前者受丝裂原活化蛋白(MAP)激酶途径调控,后者受Ras1/环AMP (cAMP)途径调控。我们之前证明,在RPMI 1640培养基中形成mtl纯合的成熟(48 h)性生物膜时,MAP激酶途径靶向Tec1而不是Cph1,后者是相同途径的靶点,但对于不透明的细胞交配反应。在这里,我们通过比较删除Tec1对有机硅弹性体初始粘附的影响,高分辨率共聚焦显微镜评估生物膜发育48小时内的阶段和细胞表型,人类白细胞渗透和生物膜脆弱性,继续分析Tec1的作用。我们发现,尽管Tec1在有机硅弹性体的初始粘附过程中只起很小的作用,但它在酵母细胞多层层的生长、菌丝的垂直延伸和生物膜上部基质的沉积、生物膜的最终厚度、人类白细胞的渗透性和最终生物膜的完整性(即对流体流动的抵抗力)中发挥了重要作用。这些结果为正常生物膜的发育和结构提供了更详细的描述,并证实了转录因子Tec1在本文采用的生物膜模型中所起的核心作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Role of Tec1 in the development, architecture, and integrity of sexual biofilms of Candida albicans.

MTL-homozygous ( A: / A: or α/α) white cells form a complex sexual biofilm that exhibits the same architecture as that of MTL-heterozygous ( A: /α) pathogenic biofilms. However, the former is regulated by the mitogen-activated protein (MAP) kinase pathway, while the latter is regulated by the Ras1/cyclic AMP (cAMP) pathway. We previously demonstrated that in the formation of an MTL-homozygous, mature (48 h) sexual biofilm in RPMI 1640 medium, the MAP kinase pathway targets Tec1 rather than Cph1, the latter of which is the target of the same pathway, but for the opaque cell mating response. Here we continued our analysis of the role of Tec1 by comparing the effects of deleting TEC1 on initial adhesion to silicone elastomer, high-resolution confocal microscopy assessments of the stages and cellular phenotypes during the 48 h of biofilm development, human white cell penetration, and biofilm fragility. We show that although Tec1 plays only a minor role in initial adhesion to the silicone elastomer, it does play a major role in the growth of the basal yeast cell polylayer, vertical extension of hyphae and matrix deposition in the upper portion of the biofilm, final biofilm thickness, penetrability of human white blood cells, and final biofilm integrity (i.e., resistance to fluid flow). These results provide a more detailed description of normal biofilm development and architecture and confirm the central role played by the transcription factor Tec1 in the biofilm model employed here.

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来源期刊
Eukaryotic Cell
Eukaryotic Cell 生物-微生物学
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1 months
期刊介绍: Eukaryotic Cell (EC) focuses on eukaryotic microbiology and presents reports of basic research on simple eukaryotic microorganisms, such as yeasts, fungi, algae, protozoa, and social amoebae. The journal also covers viruses of these organisms and their organelles and their interactions with other living systems, where the focus is on the eukaryotic cell. Topics include: - Basic biology - Molecular and cellular biology - Mechanisms, and control, of developmental pathways - Structure and form inherent in basic biological processes - Cellular architecture - Metabolic physiology - Comparative genomics, biochemistry, and evolution - Population dynamics - Ecology
期刊最新文献
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