海带多糖对哮喘小鼠气道炎症的影响。

IF 2.3 Multidisciplinary Respiratory Medicine Pub Date : 2015-06-11 eCollection Date: 2015-01-01 DOI:10.1186/s40248-015-0017-0
Rongjun Lin, Xiaomei Liu, Yan Meng, Mei Xu, Jianping Guo
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引用次数: 20

摘要

背景:哮喘是一种严重的慢性炎症性疾病,影响全球3亿人。本研究旨在探讨海带提取物对卵清蛋白(OVA)诱导的小鼠哮喘模型的抗炎和平喘作用。方法:采用ova致敏后吸入气溶胶致敏原2周建立SPF昆明小鼠哮喘模型。在OVA吸入激发期以50 mg/kg/d灌饲海带多糖(Laminaria japonica polysaccharides, LJPS),比较其与布地奈德吸入标准治疗对哮喘的影响。测定支气管肺泡灌洗液(BALF)中炎症细胞和嗜酸性粒细胞的总量。研究肺组织的组织病理学变化并评分以确定炎症程度。检测BALF中IL-12、IL-13、TGF-β1水平及血清IgE水平。检测肺组织中IL-12、IL-13、TGF-β1的表达。结果:ova诱导的哮喘小鼠肺高度炎性浸润,嗜酸性粒细胞明显增加。经OVA处理的小鼠BALF和肺组织中IL-12水平降低,IL-13和TGF-β1水平升高,血清IgE水平升高。结论:LJPS能显著抑制哮喘小鼠气道炎症反应,调节细胞因子平衡,改善肺组织病理状况。我们的数据提示LJPS可能是一种潜在的治疗过敏性哮喘的试剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Effects of Laminaria japonica polysaccharides on airway inflammation of lungs in an asthma mouse model.

Background: Asthma is a serious chronic inflammatory disease affecting 300 million people worldwide. This aim of this study to investigate the anti-inflammatory and anti-asthmatic effects of Laminaria japonica extract in the ovalbumin (OVA)-induced mouse asthma model.

Methods: A mouse asthma model was established in SPF Kunming mice by OVA-sensitization followed by inhalation of aerosol allergen for two weeks. Laminaria japonica polysaccharides (LJPS) were given by gavage feeding at 50 mg/kg/day during OVA inhalation challenge period, and their effect on asthma was compared with the standard treatment of Budesonide inhalation. The total inflammatory cells and eosinophils in bronchoalveolar lavage fluid (BALF) were determined. Histopathological changes in lung tissue were studied and scored to determine the degree of inflammation. Levels of IL-12, IL-13, and TGF-β1 in BALF as well as serum levels of IgE were measured. Expressions of IL-12, IL-13, and TGF-β1 in lung tissues were assessed.

Results: Highly inflammatory lungs infiltrated with significant increased eosinophils were observed in OVA-induced asthmatic mice. The OVA treated mice presented with a lower level of IL-12 and higher levels of IL-13 and TGF-β1 in BALF and lung tissues, as well as an increased level of the serum IgE. Treatment with LJPS (Group B) significantly decreased the numbers of eosinophils in the BALF (P < 0.05) and alleviated lung inflammation compared to the untreated asthma mice (Group A). It also reduced the serum IgE levels, increased expression of IL-12, and decreased the expression of IL-13 and TGF-β1 in BALF and lung (Both P < 0.05) compared with the group A.

Conclusions: LJPS can significantly inhibit airway inflammation of asthmatic mice, adjust the balance of cytokines, and improve the pulmonary histopathological condition. Our data suggested that LJPS might be a potential therapeutic reagent for allergic asthma.

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来源期刊
Multidisciplinary Respiratory Medicine
Multidisciplinary Respiratory Medicine Medicine-Pulmonary and Respiratory Medicine
自引率
0.00%
发文量
23
期刊介绍: Multidisciplinary Respiratory Medicine is the official journal of the Italian Respiratory Society - Società Italiana di Pneumologia (IRS/SIP). The journal publishes on all aspects of respiratory medicine and related fields, with a particular focus on interdisciplinary and translational research. The interdisciplinary nature of the journal provides a unique opportunity for researchers, clinicians and healthcare professionals across specialties to collaborate and exchange information. The journal provides a high visibility platform for the publication and dissemination of top quality original scientific articles, reviews and important position papers documenting clinical and experimental advances.
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