Fernanda Almeida-Oliveira , Samara Santos-Araujo , Luiz Fernando Carvalho-Kelly , Alessa Macedo-Silva , José Roberto Meyer-Fernandes , Katia C. Gondim , David Majerowicz
{"title":"ATP合酶对吻蝽脂质代谢的影响超出了其能量代谢的作用","authors":"Fernanda Almeida-Oliveira , Samara Santos-Araujo , Luiz Fernando Carvalho-Kelly , Alessa Macedo-Silva , José Roberto Meyer-Fernandes , Katia C. Gondim , David Majerowicz","doi":"10.1016/j.ibmb.2023.103956","DOIUrl":null,"url":null,"abstract":"<div><p><span><span>ATP synthase plays an essential role in </span>mitochondrial metabolism<span><span><span>, being responsible for the production of ATP in oxidative phosphorylation. However, recent results have shown that it may also be present in the cell membrane, involved in </span>lipophorin binding to its receptors. Here, we used a functional </span>genetics<span><span> approach to investigate the roles of ATP synthase in lipid metabolism in the </span>kissing bug </span></span></span><em>Rhodnius prolixus.</em> The genome of <em>R. prolixus</em><span> encodes five nucleotide-binding domain genes of the ATP synthase α and β family, including the α and β subunits of ATP synthase (</span><em>RpATPSynα</em> and <em>RpATPSynβ</em><span>), and the catalytic and non-catalytic subunits of the vacuolar ATPase (</span><em>RpVha68</em> and <em>RpVha55</em>). These genes were expressed in all analyzed organsn highest in the ovaries, fat body and flight muscle. Feeding did not regulate the expression of ATP synthases in the posterior midgut or fat body. Furthermore, ATP synthase is present in the fat body's mitochondrial and membrane fractions. <em>RpATPSynβ</em><span> knockdown by RNAi<span> impaired ovarian development and reduced egg-laying by approximately 85%. Furthermore, the lack of RpATPSyn</span></span><em>β</em><span> increased the amount of triacylglycerol in the fat body due to increased </span><em>de novo</em><span> fatty acid synthesis<span> and reduced transfer of lipids to lipophorin. </span></span><em>RpATPSynα</em><span> knockdown had similar effects, with altered ovarian development, reduced oviposition, and triacylglycerol accumulation in the fat body. However, ATP synthases knockdown had only a slight effect on the amount of ATP in the fat body. These results support the hypothesis that ATP synthase has a direct role in lipid metabolism and lipophorin physiology, which are not directly due to changes in energy metabolism.</span></p></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":3.2000,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"ATP synthase affects lipid metabolism in the kissing bug Rhodnius prolixus beyond its role in energy metabolism\",\"authors\":\"Fernanda Almeida-Oliveira , Samara Santos-Araujo , Luiz Fernando Carvalho-Kelly , Alessa Macedo-Silva , José Roberto Meyer-Fernandes , Katia C. Gondim , David Majerowicz\",\"doi\":\"10.1016/j.ibmb.2023.103956\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><span>ATP synthase plays an essential role in </span>mitochondrial metabolism<span><span><span>, being responsible for the production of ATP in oxidative phosphorylation. However, recent results have shown that it may also be present in the cell membrane, involved in </span>lipophorin binding to its receptors. Here, we used a functional </span>genetics<span><span> approach to investigate the roles of ATP synthase in lipid metabolism in the </span>kissing bug </span></span></span><em>Rhodnius prolixus.</em> The genome of <em>R. prolixus</em><span> encodes five nucleotide-binding domain genes of the ATP synthase α and β family, including the α and β subunits of ATP synthase (</span><em>RpATPSynα</em> and <em>RpATPSynβ</em><span>), and the catalytic and non-catalytic subunits of the vacuolar ATPase (</span><em>RpVha68</em> and <em>RpVha55</em>). These genes were expressed in all analyzed organsn highest in the ovaries, fat body and flight muscle. Feeding did not regulate the expression of ATP synthases in the posterior midgut or fat body. Furthermore, ATP synthase is present in the fat body's mitochondrial and membrane fractions. <em>RpATPSynβ</em><span> knockdown by RNAi<span> impaired ovarian development and reduced egg-laying by approximately 85%. Furthermore, the lack of RpATPSyn</span></span><em>β</em><span> increased the amount of triacylglycerol in the fat body due to increased </span><em>de novo</em><span> fatty acid synthesis<span> and reduced transfer of lipids to lipophorin. </span></span><em>RpATPSynα</em><span> knockdown had similar effects, with altered ovarian development, reduced oviposition, and triacylglycerol accumulation in the fat body. However, ATP synthases knockdown had only a slight effect on the amount of ATP in the fat body. These results support the hypothesis that ATP synthase has a direct role in lipid metabolism and lipophorin physiology, which are not directly due to changes in energy metabolism.</span></p></div>\",\"PeriodicalId\":330,\"journal\":{\"name\":\"Insect Biochemistry and Molecular Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2023-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Insect Biochemistry and Molecular Biology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0965174823000504\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Insect Biochemistry and Molecular Biology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0965174823000504","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
ATP synthase affects lipid metabolism in the kissing bug Rhodnius prolixus beyond its role in energy metabolism
ATP synthase plays an essential role in mitochondrial metabolism, being responsible for the production of ATP in oxidative phosphorylation. However, recent results have shown that it may also be present in the cell membrane, involved in lipophorin binding to its receptors. Here, we used a functional genetics approach to investigate the roles of ATP synthase in lipid metabolism in the kissing bug Rhodnius prolixus. The genome of R. prolixus encodes five nucleotide-binding domain genes of the ATP synthase α and β family, including the α and β subunits of ATP synthase (RpATPSynα and RpATPSynβ), and the catalytic and non-catalytic subunits of the vacuolar ATPase (RpVha68 and RpVha55). These genes were expressed in all analyzed organsn highest in the ovaries, fat body and flight muscle. Feeding did not regulate the expression of ATP synthases in the posterior midgut or fat body. Furthermore, ATP synthase is present in the fat body's mitochondrial and membrane fractions. RpATPSynβ knockdown by RNAi impaired ovarian development and reduced egg-laying by approximately 85%. Furthermore, the lack of RpATPSynβ increased the amount of triacylglycerol in the fat body due to increased de novo fatty acid synthesis and reduced transfer of lipids to lipophorin. RpATPSynα knockdown had similar effects, with altered ovarian development, reduced oviposition, and triacylglycerol accumulation in the fat body. However, ATP synthases knockdown had only a slight effect on the amount of ATP in the fat body. These results support the hypothesis that ATP synthase has a direct role in lipid metabolism and lipophorin physiology, which are not directly due to changes in energy metabolism.
期刊介绍:
This international journal publishes original contributions and mini-reviews in the fields of insect biochemistry and insect molecular biology. Main areas of interest are neurochemistry, hormone and pheromone biochemistry, enzymes and metabolism, hormone action and gene regulation, gene characterization and structure, pharmacology, immunology and cell and tissue culture. Papers on the biochemistry and molecular biology of other groups of arthropods are published if of general interest to the readership. Technique papers will be considered for publication if they significantly advance the field of insect biochemistry and molecular biology in the opinion of the Editors and Editorial Board.