Viktor Bubenshchikov, Artur Makichyan, Anton Larenkov
{"title":"锆-89溶液配方对[89 Zr]Zr-去铁胺合成效率的影响。","authors":"Viktor Bubenshchikov, Artur Makichyan, Anton Larenkov","doi":"10.1002/jlcr.4003","DOIUrl":null,"url":null,"abstract":"<p><p>In recent years, clinical imaging with <sup>89</sup> Zr-based radiopharmaceuticals has been gaining significant importance in nuclear medicine. This article provides the results of a comparative study of methods for obtaining <sup>89</sup> Zr solutions using ZR, Chelex-100, and TBP resins in the form of [<sup>89</sup> Zr]Zr-oxalate, [<sup>89</sup> Zr]Zr-chloride, and [<sup>89</sup> Zr]Zr-citrate in terms of purification and labeling efficiency. All evaluated methods allowed us to obtain <sup>89</sup> Zr with high yield (>90% in 1 ml). The chemical form of <sup>89</sup> Zr has a significant influence on the radiolabeling efficiency of the deferoxamine (DFO) chelator and its derivatives. Compared with [<sup>89</sup> Zr]Zr-oxalate, the application of [<sup>89</sup> Zr]Zr-chloride and [<sup>89</sup> Zr]Zr-citrate solutions leads to a higher efficacy of [<sup>89</sup> Zr]Zr-DFO complex formation. It should be noted that the sequence of mixing of the reagents during the radiolabeling reaction and the residual concentration of oxalic acid appeared to be crucial in the case of [<sup>89</sup> Zr]Zr-chloride. According to the experimental data, radiolabeling of DFO and its derivatives is preferable to use more stable chemical forms of <sup>89</sup> Zr, such as [<sup>89</sup> Zr]Zr-citrate. The concept will be applied in the further studies involving antibody-based bioconjugates.</p>","PeriodicalId":16288,"journal":{"name":"Journal of labelled compounds & radiopharmaceuticals","volume":null,"pages":null},"PeriodicalIF":0.9000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The impact of zirconium-89 solution formulation on the efficiency of [<sup>89</sup> Zr]Zr-deferoxamine synthesis.\",\"authors\":\"Viktor Bubenshchikov, Artur Makichyan, Anton Larenkov\",\"doi\":\"10.1002/jlcr.4003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In recent years, clinical imaging with <sup>89</sup> Zr-based radiopharmaceuticals has been gaining significant importance in nuclear medicine. This article provides the results of a comparative study of methods for obtaining <sup>89</sup> Zr solutions using ZR, Chelex-100, and TBP resins in the form of [<sup>89</sup> Zr]Zr-oxalate, [<sup>89</sup> Zr]Zr-chloride, and [<sup>89</sup> Zr]Zr-citrate in terms of purification and labeling efficiency. All evaluated methods allowed us to obtain <sup>89</sup> Zr with high yield (>90% in 1 ml). The chemical form of <sup>89</sup> Zr has a significant influence on the radiolabeling efficiency of the deferoxamine (DFO) chelator and its derivatives. Compared with [<sup>89</sup> Zr]Zr-oxalate, the application of [<sup>89</sup> Zr]Zr-chloride and [<sup>89</sup> Zr]Zr-citrate solutions leads to a higher efficacy of [<sup>89</sup> Zr]Zr-DFO complex formation. It should be noted that the sequence of mixing of the reagents during the radiolabeling reaction and the residual concentration of oxalic acid appeared to be crucial in the case of [<sup>89</sup> Zr]Zr-chloride. According to the experimental data, radiolabeling of DFO and its derivatives is preferable to use more stable chemical forms of <sup>89</sup> Zr, such as [<sup>89</sup> Zr]Zr-citrate. The concept will be applied in the further studies involving antibody-based bioconjugates.</p>\",\"PeriodicalId\":16288,\"journal\":{\"name\":\"Journal of labelled compounds & radiopharmaceuticals\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2022-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of labelled compounds & radiopharmaceuticals\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1002/jlcr.4003\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/10/8 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of labelled compounds & radiopharmaceuticals","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1002/jlcr.4003","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/10/8 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
The impact of zirconium-89 solution formulation on the efficiency of [89 Zr]Zr-deferoxamine synthesis.
In recent years, clinical imaging with 89 Zr-based radiopharmaceuticals has been gaining significant importance in nuclear medicine. This article provides the results of a comparative study of methods for obtaining 89 Zr solutions using ZR, Chelex-100, and TBP resins in the form of [89 Zr]Zr-oxalate, [89 Zr]Zr-chloride, and [89 Zr]Zr-citrate in terms of purification and labeling efficiency. All evaluated methods allowed us to obtain 89 Zr with high yield (>90% in 1 ml). The chemical form of 89 Zr has a significant influence on the radiolabeling efficiency of the deferoxamine (DFO) chelator and its derivatives. Compared with [89 Zr]Zr-oxalate, the application of [89 Zr]Zr-chloride and [89 Zr]Zr-citrate solutions leads to a higher efficacy of [89 Zr]Zr-DFO complex formation. It should be noted that the sequence of mixing of the reagents during the radiolabeling reaction and the residual concentration of oxalic acid appeared to be crucial in the case of [89 Zr]Zr-chloride. According to the experimental data, radiolabeling of DFO and its derivatives is preferable to use more stable chemical forms of 89 Zr, such as [89 Zr]Zr-citrate. The concept will be applied in the further studies involving antibody-based bioconjugates.
期刊介绍:
The Journal of Labelled Compounds and Radiopharmaceuticals publishes all aspects of research dealing with labeled compound preparation and applications of these compounds. This includes tracer methods used in medical, pharmacological, biological, biochemical and chemical research in vitro and in vivo.
The Journal of Labelled Compounds and Radiopharmaceuticals devotes particular attention to biomedical research, diagnostic and therapeutic applications of radiopharmaceuticals, covering all stages of development from basic metabolic research and technological development to preclinical and clinical studies based on physically and chemically well characterized molecular structures, coordination compounds and nano-particles.