Bailey M Pehde, Devon Niewohner, Faithe E Keomanivong, Michael D Carruthers
{"title":"不动杆菌噬菌体DMU1的基因组序列与特性研究。","authors":"Bailey M Pehde, Devon Niewohner, Faithe E Keomanivong, Michael D Carruthers","doi":"10.1089/phage.2020.0043","DOIUrl":null,"url":null,"abstract":"<p><p><b><i>Background:</i></b> There has been a recent resurgence of research on the characterization of <i>Acinetobacter</i> phage for therapeutic use due to the morbidity and mortality associated with treatment failures in cases of multidrug-resistant <i>Acinetobacter baumannii</i> infections. <b><i>Materials and Methods:</i></b> A bacteriophage isolated from activated sludge that targets <i>A. baumannii</i> ATCC19606 was characterized by electron microscopy, genome sequencing, comparative genomics, and a host range analysis. <b><i>Results:</i></b> The morphology of <i>Acinetobacter</i> phage DMU1 resembles phages in <i>Siphoviridae</i>. Comparative genomic and phylogenetic analyses reveal that DMU1 is a siphophage and is most closely related to <i>Acinetobacter</i> phage SH-Ab 15497. Out of the strains tested, DMU1 was found to only infect <i>A. baumannii</i> strains ATCC19606 and ATCC17978. <b><i>Conclusion:</i></b> <i>Acinetobacter</i> phage DMU1 belongs to the <i>Siphoviridae</i> family and is most closely related to <i>Acinetobacter</i> phage SH-Ab 15497. Small-scale host-range analysis of DMU1 indicates a host range that is likely limited to specific <i>A. baumannii</i> strains.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"2 1","pages":"50-56"},"PeriodicalIF":0.0000,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9041440/pdf/phage.2020.0043.pdf","citationCount":"0","resultStr":"{\"title\":\"Genome Sequence and Characterization of <i>Acinetobacter</i> Phage DMU1.\",\"authors\":\"Bailey M Pehde, Devon Niewohner, Faithe E Keomanivong, Michael D Carruthers\",\"doi\":\"10.1089/phage.2020.0043\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b><i>Background:</i></b> There has been a recent resurgence of research on the characterization of <i>Acinetobacter</i> phage for therapeutic use due to the morbidity and mortality associated with treatment failures in cases of multidrug-resistant <i>Acinetobacter baumannii</i> infections. <b><i>Materials and Methods:</i></b> A bacteriophage isolated from activated sludge that targets <i>A. baumannii</i> ATCC19606 was characterized by electron microscopy, genome sequencing, comparative genomics, and a host range analysis. <b><i>Results:</i></b> The morphology of <i>Acinetobacter</i> phage DMU1 resembles phages in <i>Siphoviridae</i>. Comparative genomic and phylogenetic analyses reveal that DMU1 is a siphophage and is most closely related to <i>Acinetobacter</i> phage SH-Ab 15497. Out of the strains tested, DMU1 was found to only infect <i>A. baumannii</i> strains ATCC19606 and ATCC17978. <b><i>Conclusion:</i></b> <i>Acinetobacter</i> phage DMU1 belongs to the <i>Siphoviridae</i> family and is most closely related to <i>Acinetobacter</i> phage SH-Ab 15497. Small-scale host-range analysis of DMU1 indicates a host range that is likely limited to specific <i>A. baumannii</i> strains.</p>\",\"PeriodicalId\":74428,\"journal\":{\"name\":\"PHAGE (New Rochelle, N.Y.)\",\"volume\":\"2 1\",\"pages\":\"50-56\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9041440/pdf/phage.2020.0043.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PHAGE (New Rochelle, N.Y.)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1089/phage.2020.0043\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2021/3/17 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PHAGE (New Rochelle, N.Y.)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/phage.2020.0043","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/3/17 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
Genome Sequence and Characterization of Acinetobacter Phage DMU1.
Background: There has been a recent resurgence of research on the characterization of Acinetobacter phage for therapeutic use due to the morbidity and mortality associated with treatment failures in cases of multidrug-resistant Acinetobacter baumannii infections. Materials and Methods: A bacteriophage isolated from activated sludge that targets A. baumannii ATCC19606 was characterized by electron microscopy, genome sequencing, comparative genomics, and a host range analysis. Results: The morphology of Acinetobacter phage DMU1 resembles phages in Siphoviridae. Comparative genomic and phylogenetic analyses reveal that DMU1 is a siphophage and is most closely related to Acinetobacter phage SH-Ab 15497. Out of the strains tested, DMU1 was found to only infect A. baumannii strains ATCC19606 and ATCC17978. Conclusion:Acinetobacter phage DMU1 belongs to the Siphoviridae family and is most closely related to Acinetobacter phage SH-Ab 15497. Small-scale host-range analysis of DMU1 indicates a host range that is likely limited to specific A. baumannii strains.
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