高静水联合碱性电解水处理对细菌孢子损伤及生长特性的影响。

IF 0.9 4区 农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biocontrol science Pub Date : 2022-01-01 DOI:10.4265/bio.27.131
Daisuke Hamanaka, Koki Morita
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引用次数: 0

摘要

研究了高静水处理(HPP)加/不加碱性电解水(AlEW)降低枯草芽孢杆菌(Bacillus subtilis)耐热性后芽孢的生长和损伤特性。当与AlEW联合使用时,生长曲线滞后期的延迟,特别是在50 MPa或更小的情况下,延长了约4至6小时。然而,处理过程中的温度和时间对滞后期的影响在单独hpp和联合AlEW之间没有显著差异。用抗生素(青霉素G、利福平和氯霉素)添加胰酶豆汤,评价处理后细菌孢子的损伤特征。在添加氯霉素的TSB中,虽然与正常TSB相比,HPP与AlEW处理的细菌孢子的滞后期没有延长,但在对数期生长速度下降,最大生长量增加。这一结果可能是由于HPP与AlEW联合治疗造成的损伤,如抑制蛋白质合成。此外,与AlEW联合处理在30、80和100 MPa下,过氧化氢酶活性分别比单独hpp处理提高了1.45倍、1.63倍和4.25倍,因此,联合处理可能对细菌孢子产生较高的氧化应激。
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Effect of High Hydrostatic Pressure Treatment Combined with Alkaline Electrolyzed Water on the Injury and Growth Characteristics of Bacterial Spores.

The characteristics of growth and injury of Bacillus subtilis spores whose heat resistance was reduced by the treatment of high hydrostatic pressure processing (HPP) combined with/without alkaline electrolyzed water (AlEW) were investigated. The delay in the lag phase of growth curve observed when used in combination with AlEW, especially at 50 MPa or less, and was prolonged by about 4 to 6 hours. However, the effects of temperature and time during treatment on the lag phase were not significantly different between solo-HPP and in combination with AlEW. The injury characteristics of the treated bacterial spores were evaluated by using antibiotics (penicillin G, rifampicin and chloramphenicol) supplemented trypticase soy broth. In the chloramphenicol supplemented TSB, although the lag phase of bacterial spores treated by HPP with AlEW was not prolonged as compared with the normal-TSB, the decrease in growth rate during logarithmic phase and increase in maximum growth amount were observed. This result could suggest due to a damage by combined treatment of HPP with AlEW such as the inhibition of protein synthesis. Furthermore, the combined treatment with AlEW increased the catalase activity by 1.45, 1.63 and 4.25 times at 30, 80 and 100 MPa, respectively, as compared with solo-HPP, therefore the combined treatment could cause high oxidative stress on bacterial spores.

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来源期刊
Biocontrol science
Biocontrol science BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
2.60
自引率
8.30%
发文量
21
审稿时长
>12 weeks
期刊介绍: The Biocontrol Science provides a medium for the publication of original articles, concise notes, and review articles on all aspects of science and technology of biocontrol.
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