{"title":"低温原液对细菌生长的影响。","authors":"Mitsuyo Kita, Jun Usukura","doi":"10.4265/bio.27.163","DOIUrl":null,"url":null,"abstract":"<p><p>It is necessary to know the appropriate bacterial culture temperature and stock temperature to carry out high-precision preservative efficacy tests. In this study, we investigated whether the temperature conditions within the culture or stock affect the survivability or growth activity of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. We found that the cold stocking condition affected the survivability of P. aeruginosa, but not that of E. coli and S. aureus. Furthermore, stocking temperature affects the growth activity of E. coli, P. aeruginosa and S. aureus, and the effect on P. aeruginosa was greater than that on the others. However, the attenuating effect on P. aeruginosa was reversed by culturing into fresh medium for up to six weeks after stocking. These results suggest that cold stress during stocking may affect the growth activity of P. aeruginosa. The results of this study should provide knowledge for the precise evaluation of preservative efficacy in cosmetics.</p>","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":null,"pages":null},"PeriodicalIF":0.9000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Low-temperature Stock on the Growth of Bacteria.\",\"authors\":\"Mitsuyo Kita, Jun Usukura\",\"doi\":\"10.4265/bio.27.163\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>It is necessary to know the appropriate bacterial culture temperature and stock temperature to carry out high-precision preservative efficacy tests. In this study, we investigated whether the temperature conditions within the culture or stock affect the survivability or growth activity of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. We found that the cold stocking condition affected the survivability of P. aeruginosa, but not that of E. coli and S. aureus. Furthermore, stocking temperature affects the growth activity of E. coli, P. aeruginosa and S. aureus, and the effect on P. aeruginosa was greater than that on the others. However, the attenuating effect on P. aeruginosa was reversed by culturing into fresh medium for up to six weeks after stocking. These results suggest that cold stress during stocking may affect the growth activity of P. aeruginosa. The results of this study should provide knowledge for the precise evaluation of preservative efficacy in cosmetics.</p>\",\"PeriodicalId\":8777,\"journal\":{\"name\":\"Biocontrol science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biocontrol science\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.4265/bio.27.163\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biocontrol science","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.4265/bio.27.163","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Effect of Low-temperature Stock on the Growth of Bacteria.
It is necessary to know the appropriate bacterial culture temperature and stock temperature to carry out high-precision preservative efficacy tests. In this study, we investigated whether the temperature conditions within the culture or stock affect the survivability or growth activity of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. We found that the cold stocking condition affected the survivability of P. aeruginosa, but not that of E. coli and S. aureus. Furthermore, stocking temperature affects the growth activity of E. coli, P. aeruginosa and S. aureus, and the effect on P. aeruginosa was greater than that on the others. However, the attenuating effect on P. aeruginosa was reversed by culturing into fresh medium for up to six weeks after stocking. These results suggest that cold stress during stocking may affect the growth activity of P. aeruginosa. The results of this study should provide knowledge for the precise evaluation of preservative efficacy in cosmetics.
期刊介绍:
The Biocontrol Science provides a medium for the publication of original articles, concise notes, and review articles on all aspects of science and technology of biocontrol.