应用DiVSaL方法评价致死应激后细菌孢子损伤群体的理论基础。

IF 0.9 4区 农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biocontrol science Pub Date : 2022-01-01 DOI:10.4265/bio.27.169
Ryoko Asada, Jin J Sakamoto, Masakazu Furuta, Tetsuaki Tsuchido
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引用次数: 1

摘要

为了评估暴露于致死胁迫下的细菌孢子群体的损伤,我们提出了应用DiVSaL方法的理论基础,该方法已经被报道为一般微生物的双重传代培养方法。我们建立了一个数学模型,同时考虑了对萌发系统和孢子体的伤害。在这一理论中,我们合理地假设在随后的营养生长之前,存活和可发芽的孢子数是恒定的,并且发芽和生长的延迟可以包括在λ伤害的概念中,之前报道的是生长无关的伤害。通过引入这些假设,可以认为双重传代培养方法也适用于孢子。作为应用这一理论的例子,分析了加热和紫外线照射处理枯草芽孢杆菌孢子的生长延迟,并评估了损伤孢子的数量。根据所获得的结果,表明热量比紫外线辐射具有更高的伤害产生能力。介绍了DiVSaL方法作为食品保鲜和卫生设计工具的适用性。
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Theoretical Base for the Application of the DiVSaL Method to Bacterial Spores to Evaluate Injured Populations Occurring After Exposure to Lethal Stress.

To assess injury in bacterial spore populations exposed to lethal stress, we proposed a theoretical basis for applying the DiVSaL method, which has already been reported for general microorganisms as a double subculture method. We constructed a mathematical model in which both injuries to the germination system and the spore body were taken into the theory. In this theory, we reasonably assumed that the viable and germinable spore count is constant before the subsequent vegetative growth and that the delay of germination and outgrowth can be included in the concept of λ injury previously reported as the growth-independent injury. By introducing these assumptions, the double subculture method can be considered to apply to spores as well. As examples of the application of this theory, the growth delays of Bacillus subtilis spores treated with heat and UV irradiation were analyzed and the numbers of injured spores were evaluated. Based on the results obtained, heat is indicated to have a higher injury generation ability than UV irradiation. The applicability of the DiVSaL method as a tool for food preservation and sanitation designs is presented.

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来源期刊
Biocontrol science
Biocontrol science BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
2.60
自引率
8.30%
发文量
21
审稿时长
>12 weeks
期刊介绍: The Biocontrol Science provides a medium for the publication of original articles, concise notes, and review articles on all aspects of science and technology of biocontrol.
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