血小板是否抑制阿司匹林对癌细胞的作用?

Q2 Medicine Cancer Microenvironment Pub Date : 2015-08-01 Epub Date: 2015-07-04 DOI:10.1007/s12307-015-0169-7
Neha Mehta, Sasikala Muthusamy, Alka Bhatia
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引用次数: 1

摘要

血小板和癌细胞都表现出一种亲密的相互串扰,导致彼此特性的改变。尽管过去的许多研究都试图证明血小板对肿瘤细胞的作用,但血小板在癌变中的确切作用仍不清楚。在上述研究中,我们探讨了不同浓度富血小板血浆(PRP)在培养条件下对HeLa细胞活力、增殖和粘附的影响。上述参数在低浓度PRP (4.4 × 10(5)和1 × 10(6)个血小板/μl)孵育时略有增加,而在高浓度PRP (2 × 10(6)个血小板/μl)孵育24 h时则相反。为了进一步证实上述影响是由血小板引起的,我们在抗血小板药物阿司匹林(20 mM)存在的情况下重复了实验。单独使用阿司匹林治疗时,细胞活力、增殖和粘附能力下降,表明阿司匹林对HeLa细胞具有细胞毒性。然而,在添加所有PRP浓度24小时后,发现上述所有参数都增加了。总的来说,血小板数量的变化对癌细胞产生了不同的影响。阿司匹林的使用降低了癌细胞的活力,但这种效果被所有使用的PRP浓度部分逆转。
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Do Platelets Inhibit the Effect of Aspirin on Cancer Cells?

Both platelets and cancer cells display an intimate reciprocal crosstalk resulting in alteration of each other's properties. Although many past studies have tried to demonstrate effect of platelets on tumour cells, exact role of platelets in carcinogenesis is still not clear. In the above study, we explored the effect of different concentrations of platelet rich plasma (PRP) on viability, proliferation and adhesion of HeLa cells in culture conditions. The above parameters were found to be slightly increased on incubation with lower two concentrations of PRP (4.4 × 10(5) & 1 × 10(6) platelets/μl) while a reverse effect was seen at high PRP concentration (2 × 10(6) lac platelets/μl) especially at 24 h. To further validate that the above effects were due to platelets we repeated the experiments in the presence of antiplatelet drug aspirin (20 mM). On treatment with aspirin alone, the cell viability, proliferation and adhesion were seen to be decreased indicating cytotoxicity of aspirin towards HeLa cells. However, all of the above parameters were found to increase on addition of all PRP concentrations at 24 h. Overall, variations in the number of platelets produced different effects on the cancer cells. Use of aspirin reduced the viability of the cancer cells, but this effect was seen to be partially reversed by all the concentrations of PRP used.

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来源期刊
Cancer Microenvironment
Cancer Microenvironment Medicine-Oncology
CiteScore
4.90
自引率
0.00%
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0
期刊介绍: Cancer Microenvironment is the official journal of the International Cancer Microenvironment Society (ICMS). It publishes original studies in all aspects of basic, clinical and translational research devoted to the study of cancer microenvironment. It also features reports on clinical trials. Coverage in Cancer Microenvironment includes: regulation of gene expression in the cancer microenvironment; innate and adaptive immunity in the cancer microenvironment, inflammation and cancer; tumor-associated stroma and extracellular matrix, tumor-endothelium interactions (angiogenesis, extravasation), cancer stem cells, the metastatic niche, targeting the tumor microenvironment: preclinical and clinical trials.
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