DNA微阵列研究发现山葵衍生异硫氰酸盐对IMR-32细胞nrf2介导的神经元保护作用

Gene regulation and systems biology Pub Date : 2016-08-11 eCollection Date: 2016-01-01 DOI:10.4137/GRSB.S39440
Phoebe Zapanta Trio, Satoru Fujisaki, Shunsuke Tanigawa, Ayami Hisanaga, Kozue Sakao, De-Xing Hou
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引用次数: 18

摘要

6-(甲基亚砜基)己基异硫氰酸酯(6- msitc)、6-(甲基亚砜基)己基异硫氰酸酯(6- mtitc)和4-(甲基亚砜基)丁基异硫氰酸酯(4- msitc)是从芥末中提取的异硫氰酸酯(ITC)生物活性化合物。先前的体内研究强调了ITCs的神经保护潜力,因为ITCs可以促进抗氧化剂相关酶的产生。因此,在本研究中,设计了全基因组DNA微阵列分析,以描述这些ITCs刺激神经元细胞系IMR-32的基因表达变化。在这些ITCs中,6-MSITC引起了大多数基因的表达变化(263个),其中100个基因表达上调,163个基因表达下调。基因分类显示大部分差异表达基因参与氧化应激反应,通路分析进一步揭示nrf2介导的氧化应激通路是itc调控的信号通路的顶端。最后通过实时聚合酶链反应(real-time polymerase chain reaction, PCR)和Western blotting对主要靶点的基因表达和蛋白产物进行验证。综上所述,芥末衍生的ITCs可能针对nrf2介导的氧化应激途径发挥神经保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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DNA Microarray Highlights Nrf2-Mediated Neuron Protection Targeted by Wasabi-Derived Isothiocyanates in IMR-32 Cells.

6-(Methylsulfinyl)hexyl isothiocyanate (6-MSITC), 6-(methylthio)hexyl isothiocyanate (6-MTITC), and 4-(methylsulfinyl)butyl isothiocyanate (4-MSITC) are isothiocyanate (ITC) bioactive compounds from Japanese Wasabi. Previous in vivo studies highlighted the neuroprotective potential of ITCs since ITCs enhance the production of antioxidant-related enzymes. Thus, in this present study, a genome-wide DNA microarray analysis was designed to profile gene expression changes in a neuron cell line, IMR-32, stimulated by these ITCs. Among these ITCs, 6-MSITC caused the expression changes of most genes (263), of which 100 genes were upregulated and 163 genes were downregulated. Gene categorization showed that most of the differentially expressed genes are involved in oxidative stress response, and pathway analysis further revealed that Nrf2-mediated oxidative stress pathway is the top of the ITC-modulated signaling pathway. Finally, real-time polymerase chain reaction (PCR) and Western blotting confirmed the gene expression and protein products of the major targets by ITCs. Taken together, Wasabi-derived ITCs might target the Nrf2-mediated oxidative stress pathway to exert neuroprotective effects.

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