储存对炭疽芽孢杆菌环境表面采样标本完整性的影响。

Biosafety Pub Date : 2013-01-01 Epub Date: 2013-01-17 DOI:10.4172/2167-0331.S1-002
K Allison Perry, Heather A O'Connell, Laura J Rose, Judith A Noble-Wang, Matthew J Arduino
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引用次数: 6

摘要

包装、运输温度和贮存时间对炭疽芽孢杆菌恢复的影响。研究了拭子中的孢子。将Macrofoam拭子预湿润,接种炭疽芽孢杆菌孢子,包装在一级容器或二级容器中,然后在-15°C、5°C、21°C或35°C条件下保存0-7天。根据经过验证的疾病控制中心/实验室反应网络培养方案处理拭子,并确定每个变量相对于参考样本的回收率(T0)。在-15°C和5°C条件下,拭子的恢复无差异(p≥0.23)。这两种温度比21°C或35°C温度下的拭子回收率显著提高(共7天,p≤0.04)。如果在第1、2或4天处理,在5°C下的回收率没有显著差异,但在第7天显著降低(第2天vs. 7,5°C, 102, p=0.03)。无论储存时间如何(5°C数据,p≤0.008),二次收容提供的回收率明显高于一次收容。在-15°C或5°C条件下保存,并在4天内进行处理,以产生最佳的孢子回收率,可保持装运的含有炭疽芽孢杆菌孢子的环境棉签样本的完整性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Storage Effects on Sample Integrity of Environmental Surface Sampling Specimens with Bacillus anthracis Spores.

The effect of packaging, shipping temperatures and storage times on recovery of Bacillus anthracis. Sterne spores from swabs was investigated. Macrofoam swabs were pre-moistened, inoculated with Bacillus anthracis spores, and packaged in primary containment or secondary containment before storage at -15°C, 5°C, 21°C, or 35°C for 0-7 days. Swabs were processed according to validated Centers for Disease Control/Laboratory Response Network culture protocols, and the percent recovery relative to a reference sample (T0) was determined for each variable. No differences were observed in recovery between swabs held at -15° and 5°C, (p ≥ 0.23). These two temperatures provided significantly better recovery than swabs held at 21°C or 35°C (all 7 days pooled, p ≤ 0.04). The percent recovery at 5°C was not significantly different if processed on days 1, 2 or 4, but was significantly lower on day 7 (day 2 vs. 7, 5°C, 102, p=0.03). Secondary containment provided significantly better percent recovery than primary containment, regardless of storage time (5°C data, p ≤ 0.008). The integrity of environmental swab samples containing Bacillus anthracis spores shipped in secondary containment was maintained when stored at -15°C or 5°C and processed within 4 days to yield the optimum percent recovery of spores.

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Storage Effects on Sample Integrity of Environmental Surface Sampling Specimens with Bacillus anthracis Spores.
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