{"title":"维甲酸对大鼠子宫mmp-2生成、增殖及超微结构形态的影响","authors":"Tuğba Kotil , Şengül Şentürk , Türkan Sarioğlu , Leyla Tapul","doi":"10.1016/j.etp.2017.06.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Aims</h3><p>Retinoic acid<span><span> (RA) has a vital importance in order to ensure continuity and morphology in many tissues. Matrix metalloproteinases (MMPs) have significant roles in proliferation, the formation of cancers, and </span>metastasis. In this study the effects of RA on MMP-2 production in cells of rat uterus were investigated.</span></p></div><div><h3>Methods</h3><p>Twenty-four adult Spraque Dawley rats were divided into two groups, the experimental group was treated with 40<!--> <!-->mg/kg/day 13-cis RA for 5<!--> <span><span><span>days by gavage. Uterine tissue sections were treated with </span>BrdU and MMP-2 antibodies, evaluated using </span>light microscopy<span>. Tissues were fixed with 2.5% glutaraldehyde and evaluated using transmission electron miroscopy.</span></span></p></div><div><h3>Results</h3><p>MMP-2 immunoreactivity<span><span><span><span> decreased in the stromal cells compared with the control group and no staining of MMP-2 was observed in glandular epithelium in the experimental group. BrDU labeling of cells showed significant decrease in RA-treated group versus control group cells. Based on the </span>electron microscopy evaluation, the surface epithelial cells of the experimental group showed </span>vacuolization<span>, and an accumulation of lipofuscin<span> bodies was also observed in the gland epithelium. Cells involving autophagic vacuoles contained excess lipid granules in the entire uterus layers especially localized at the border of the endometrium and </span></span></span>myometrium.</span></p></div><div><h3>Conclusion</h3><p>RA had negative effects on cell proliferation and cell morphology and inhibited MMP-2 expression.</p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 8","pages":"Pages 643-648"},"PeriodicalIF":0.0000,"publicationDate":"2017-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.06.002","citationCount":"2","resultStr":"{\"title\":\"The effects of retinoic acid on mmp-2 production, proliferation and ultrastructural morphology in rat uterus\",\"authors\":\"Tuğba Kotil , Şengül Şentürk , Türkan Sarioğlu , Leyla Tapul\",\"doi\":\"10.1016/j.etp.2017.06.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Aims</h3><p>Retinoic acid<span><span> (RA) has a vital importance in order to ensure continuity and morphology in many tissues. Matrix metalloproteinases (MMPs) have significant roles in proliferation, the formation of cancers, and </span>metastasis. In this study the effects of RA on MMP-2 production in cells of rat uterus were investigated.</span></p></div><div><h3>Methods</h3><p>Twenty-four adult Spraque Dawley rats were divided into two groups, the experimental group was treated with 40<!--> <!-->mg/kg/day 13-cis RA for 5<!--> <span><span><span>days by gavage. Uterine tissue sections were treated with </span>BrdU and MMP-2 antibodies, evaluated using </span>light microscopy<span>. Tissues were fixed with 2.5% glutaraldehyde and evaluated using transmission electron miroscopy.</span></span></p></div><div><h3>Results</h3><p>MMP-2 immunoreactivity<span><span><span><span> decreased in the stromal cells compared with the control group and no staining of MMP-2 was observed in glandular epithelium in the experimental group. BrDU labeling of cells showed significant decrease in RA-treated group versus control group cells. Based on the </span>electron microscopy evaluation, the surface epithelial cells of the experimental group showed </span>vacuolization<span>, and an accumulation of lipofuscin<span> bodies was also observed in the gland epithelium. Cells involving autophagic vacuoles contained excess lipid granules in the entire uterus layers especially localized at the border of the endometrium and </span></span></span>myometrium.</span></p></div><div><h3>Conclusion</h3><p>RA had negative effects on cell proliferation and cell morphology and inhibited MMP-2 expression.</p></div>\",\"PeriodicalId\":50465,\"journal\":{\"name\":\"Experimental and Toxicologic Pathology\",\"volume\":\"69 8\",\"pages\":\"Pages 643-648\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-10-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.etp.2017.06.002\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental and Toxicologic Pathology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0940299316302615\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental and Toxicologic Pathology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0940299316302615","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}
The effects of retinoic acid on mmp-2 production, proliferation and ultrastructural morphology in rat uterus
Aims
Retinoic acid (RA) has a vital importance in order to ensure continuity and morphology in many tissues. Matrix metalloproteinases (MMPs) have significant roles in proliferation, the formation of cancers, and metastasis. In this study the effects of RA on MMP-2 production in cells of rat uterus were investigated.
Methods
Twenty-four adult Spraque Dawley rats were divided into two groups, the experimental group was treated with 40 mg/kg/day 13-cis RA for 5 days by gavage. Uterine tissue sections were treated with BrdU and MMP-2 antibodies, evaluated using light microscopy. Tissues were fixed with 2.5% glutaraldehyde and evaluated using transmission electron miroscopy.
Results
MMP-2 immunoreactivity decreased in the stromal cells compared with the control group and no staining of MMP-2 was observed in glandular epithelium in the experimental group. BrDU labeling of cells showed significant decrease in RA-treated group versus control group cells. Based on the electron microscopy evaluation, the surface epithelial cells of the experimental group showed vacuolization, and an accumulation of lipofuscin bodies was also observed in the gland epithelium. Cells involving autophagic vacuoles contained excess lipid granules in the entire uterus layers especially localized at the border of the endometrium and myometrium.
Conclusion
RA had negative effects on cell proliferation and cell morphology and inhibited MMP-2 expression.
期刊介绍：
Cessation. The international multidisciplinary journal is devoted to the publication of studies covering the whole range of experimental research on disease processes and toxicology including cell biological investigations. Its aim is to support progress in the interdisciplinary cooperation of researchers working in pathobiology, toxicology, and cell biology independent of the methods applied. During the past decades increasing attention has been paid to the importance of toxic influence in the pathogenesis of human and animal diseases. This is why Experimental and Toxicologic Pathology meets the urgent need for an interdisciplinary journal felt by a wide variety of experts in medicine and biology, including pathologists, toxicologists, biologists, physicians, veterinary surgeons, pharmacists, and pharmacologists working in academic, industrial or clinical institutions.
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