大麻素诱导小鼠四分体

Q2 Neuroscience Current Protocols in Neuroscience Pub Date : 2018-02-13 DOI:10.1002/cpns.31

Mathilde Metna-Laurent, Miguel Mondésir, Agnès Grel, Monique Vallée, Pier-Vincenzo Piazza

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引用次数: 54

摘要

大麻素诱导的四分体是一种临床前模型，通常用于评估一种药理化合物是否是啮齿动物中枢1型大麻素(CB1)受体的激动剂。四分体的特征是低运动、低体温、嗜睡和镇痛，这四种表型是由CB1激动剂急性给药引起的，例如原型大麻素δ -9-四氢大麻酚(THC)。本单元描述了以四氢大麻酚作为参考大麻素在小鼠中诱导四分体表型的标准方案。我们提供了用这种方法获得的典型结果，显示了四氢大麻酚在不同小鼠品系中的剂量效应。CB1拮抗剂利莫那班的作用也被证实。这种四分体方案很好地适应于揭示体内作用于CB1受体的新化合物。©2017 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Cannabinoid-Induced Tetrad in Mice

Cannabinoid-induced tetrad is a preclinical model commonly used to evaluate if a pharmacological compound is an agonist of the central type-1 cannabinoid (CB1) receptor in rodents. The tetrad is characterized by hypolocomotion, hypothermia, catalepsy, and analgesia, four phenotypes that are induced by acute administration of CB1 agonists exemplified by the prototypic cannabinoid delta-9-tetrahydrocannabinol (THC). This unit describes a standard protocol in mice to induce tetrad phenotypes with THC as reference cannabinoid. We provide typical results obtained with this procedure showing a dose effect of THC in different mouse strains. The effect of the CB1 antagonist rimonabant is also shown. This tetrad protocol is well adapted to reveal new compounds acting on CB1 receptors in vivo. © 2017 by John Wiley & Sons, Inc.

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期刊介绍： Current Protocols in Neuroscience is a one-stop resource for finding and adapting the best models and methods for all types of neuroscience experiments. Updated every three months in all formats, CPNS is constantly evolving to keep pace with the very latest discoveries and developments. A year of these quarterly updates is included in the initial CPNS purchase price.