Gang Wu, Irene C M Kwan, Zhimin Yan, Yining Huang, Eric Ye
{"title":"ph5下形成的5′-单磷酸鸟苷的螺旋结构:是左旋的还是右旋的?","authors":"Gang Wu, Irene C M Kwan, Zhimin Yan, Yining Huang, Eric Ye","doi":"10.1155/2017/6798759","DOIUrl":null,"url":null,"abstract":"<p><p>Early X-ray fiber diffraction studies have established that the spontaneous gel formation of guanosine 5'-monophosphate (5'-GMP) under slightly acidic conditions (e.g., pH 5) results from self-assembly of 5'-GMP into a helical structure in which hydrogen-bonded guanine bases form a continuous helix with 15 nucleotides per 4 turns. For more than five decades, the sense of this helix is believed to be left-handed. Using multinuclear solid-state NMR and IR spectroscopic methods, we have finally determined the long-missing structural details of this helix. First, we found that this 5'-GMP helix is right-handed containing exclusive C3'-<i>endo</i> sugar puckers. Second, we showed that the central channel of this helix is free of Na<sup>+</sup> ions, which is in sharp contrast to the helix formed by 5'-GMP at pH 8 where the central channel is filled with Na<sup>+</sup> ions.</p>","PeriodicalId":16575,"journal":{"name":"Journal of Nucleic Acids","volume":"2017 ","pages":"6798759"},"PeriodicalIF":1.3000,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/6798759","citationCount":"2","resultStr":"{\"title\":\"On the Helical Structure of Guanosine 5'-Monophosphate Formed at pH 5: Is It Left- or Right-Handed?\",\"authors\":\"Gang Wu, Irene C M Kwan, Zhimin Yan, Yining Huang, Eric Ye\",\"doi\":\"10.1155/2017/6798759\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Early X-ray fiber diffraction studies have established that the spontaneous gel formation of guanosine 5'-monophosphate (5'-GMP) under slightly acidic conditions (e.g., pH 5) results from self-assembly of 5'-GMP into a helical structure in which hydrogen-bonded guanine bases form a continuous helix with 15 nucleotides per 4 turns. For more than five decades, the sense of this helix is believed to be left-handed. Using multinuclear solid-state NMR and IR spectroscopic methods, we have finally determined the long-missing structural details of this helix. First, we found that this 5'-GMP helix is right-handed containing exclusive C3'-<i>endo</i> sugar puckers. Second, we showed that the central channel of this helix is free of Na<sup>+</sup> ions, which is in sharp contrast to the helix formed by 5'-GMP at pH 8 where the central channel is filled with Na<sup>+</sup> ions.</p>\",\"PeriodicalId\":16575,\"journal\":{\"name\":\"Journal of Nucleic Acids\",\"volume\":\"2017 \",\"pages\":\"6798759\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2017-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1155/2017/6798759\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Nucleic Acids\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/2017/6798759\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2017/11/2 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Nucleic Acids","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2017/6798759","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2017/11/2 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
On the Helical Structure of Guanosine 5'-Monophosphate Formed at pH 5: Is It Left- or Right-Handed?
Early X-ray fiber diffraction studies have established that the spontaneous gel formation of guanosine 5'-monophosphate (5'-GMP) under slightly acidic conditions (e.g., pH 5) results from self-assembly of 5'-GMP into a helical structure in which hydrogen-bonded guanine bases form a continuous helix with 15 nucleotides per 4 turns. For more than five decades, the sense of this helix is believed to be left-handed. Using multinuclear solid-state NMR and IR spectroscopic methods, we have finally determined the long-missing structural details of this helix. First, we found that this 5'-GMP helix is right-handed containing exclusive C3'-endo sugar puckers. Second, we showed that the central channel of this helix is free of Na+ ions, which is in sharp contrast to the helix formed by 5'-GMP at pH 8 where the central channel is filled with Na+ ions.