ph5下形成的5′-单磷酸鸟苷的螺旋结构:是左旋的还是右旋的?

IF 1.3 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Nucleic Acids Pub Date : 2017-01-01 Epub Date: 2017-11-02 DOI:10.1155/2017/6798759
Gang Wu, Irene C M Kwan, Zhimin Yan, Yining Huang, Eric Ye
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引用次数: 2

摘要

早期的x射线纤维衍射研究已经证实,在微酸性条件下(例如pH为5),鸟苷5′-单磷酸(5′-GMP)的自发凝胶形成是由于5′-GMP自组装成螺旋结构,其中氢键鸟嘌呤碱基形成一个连续的螺旋,每4转15个核苷酸。50多年来,这个螺旋的感觉被认为是左旋的。利用多核固体核磁共振和红外光谱方法,我们最终确定了这个螺旋的长期缺失的结构细节。首先,我们发现这个5'-GMP螺旋是右手性的,包含独占的C3'-末端糖袋。其次,我们发现该螺旋的中心通道没有Na+离子,这与pH为8时5'-GMP形成的螺旋形成鲜明对比,其中中心通道充满了Na+离子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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On the Helical Structure of Guanosine 5'-Monophosphate Formed at pH 5: Is It Left- or Right-Handed?

Early X-ray fiber diffraction studies have established that the spontaneous gel formation of guanosine 5'-monophosphate (5'-GMP) under slightly acidic conditions (e.g., pH 5) results from self-assembly of 5'-GMP into a helical structure in which hydrogen-bonded guanine bases form a continuous helix with 15 nucleotides per 4 turns. For more than five decades, the sense of this helix is believed to be left-handed. Using multinuclear solid-state NMR and IR spectroscopic methods, we have finally determined the long-missing structural details of this helix. First, we found that this 5'-GMP helix is right-handed containing exclusive C3'-endo sugar puckers. Second, we showed that the central channel of this helix is free of Na+ ions, which is in sharp contrast to the helix formed by 5'-GMP at pH 8 where the central channel is filled with Na+ ions.

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来源期刊
Journal of Nucleic Acids
Journal of Nucleic Acids BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
3.10
自引率
21.70%
发文量
5
审稿时长
12 weeks
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