[MALDI-TOF质谱法比较血培养阳性瓶预处理试剂盒]。

Takenori Yamashita
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引用次数: 0

摘要

脓毒症或菌血症的细菌鉴定是制定治疗政策的有用结果。近年来,利用MALDI-TOF对血培养瓶进行细菌鉴定已成为可能,但其准确性不及琼脂菌落鉴定。血液培养预处理试剂盒(MALDI Sepcityper kit)目前在布鲁克销售。然而,目前的情况还没有达到很好的准确性。这次出现了一种新的血培养预处理试剂盒,于是我进行了研究。目前,血培养前处理试剂盒仅有MALDI Sepcityper kit,采用酶消化法。快速BAC pro (Nittobo)是一种使用纳米材料的预处理试剂盒。本次进行对比检查(共40个样本)。其中33份标本经MALDI Sepcityper Kit鉴定。有21份标本可通过快速BAC pro进行鉴定。在这项研究中,快速BAC pro没有显示出优于MALDI Sepcityper Kit的结果。
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[MALDI-TOF MS Comparison of Blood Culture Positive Bottle Pretreatment Kit].

Identification of bacteria of sepsis or bacteremia is a useful result for treatment policy. In recent years, bacterial identification has become possible from blood culture bottles by MALDI‒TOF, but it is not as accurate as bacterial identification from agar colonies. Blood culture pretreatment kit (MALDI Sepcityper Kit) is currently on sale from Bruker. However, the current situation has not reached good accuracy. This time, a new blood culture pretreatment kit appeared, so I studied. Up to now, the blood culture pretreatment kit was only MALDI Sepcityper Kit using enzyme digestion method. Rapid BAC pro (Nittobo) is a pretreatment kit using nanomaterials. This time, comparison examination (total number 40 samples) was done. Among them, 33 specimens were identified by MALDI Sepcityper Kit. There were 21 specimens that could be identified by rapid BAC pro. In this study, rapid BAC pro did not show superior results over MALDI Sepcityper Kit.

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