Maria Giovanna Vilia, Marta Tocchetti, Eleonora Fonte, Ilenia Sana, Marta Muzio
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Characterization of a long isoform of IL-1R8 (TIR8/SIGIRR).
IL-1R8, also known as SIGIRR or TIR8, is a trans-membrane protein belonging to the IL-1 receptor family. The human gene includes ten exons, and alternative splicing can result in different isoforms. We, herein, characterized a longer isoform of IL-1R8 containing an in-frame additional sequence between the TIR domain and the C-terminal portion of the protein. IL-1R8 Long (IL-1R8L1) mRNA was specifically expressed and regulated in distinct cell lines, in a manner similar to the classic isoform. Overexpression of IL-1R8L1 resulted in the production of a corresponding protein that showed a pattern of cell localization similar to the classic isoform. An antibody directed against an IL-1R8L1 specific peptide, detected this novel isoform in different cell lines and tissues where this protein may complement the anti-inflammatory functions of classic IL-1R8.
期刊介绍：
The journal that brings together all areas of work involving cytokines.
European Cytokine Network is an electronic journal that publishes original articles and abstracts every quarter to provide an essential bridge between researchers and clinicians with an interest in this cutting-edge field.
The journal has become a must-read for specialists in the field thanks to its swift publication and international circulation.
The journal is referenced in several databases, including Medline, which is testament to its scientific quality.
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