白藜芦醇对链脲佐菌素诱导的糖尿病小鼠胰岛移植后移植血管再通的影响。

IF 1.9 4区 医学 Q3 ENDOCRINOLOGY & METABOLISM Islets Pub Date : 2018-01-02 Epub Date: 2018-01-15 DOI:10.1080/19382014.2017.1414764
Eun-Mi Lee, Inwon Park, Ye-Jee Lee, Young-Hye You, Ji-Won Kim, Myung-Jun Kim, Yu-Bae Ahn, Pilhan Kim, Seung-Hyun Ko
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摘要

我们评估了白藜芦醇(RSV)对糖尿病小鼠胰岛移植(ITx)后移植物存活率的影响。从 Balb/c 小鼠体内分离出的胰岛(200 IEQ)被移植到糖尿病 Balb/c 小鼠的肾囊下。在 ITx 移植后的 14 天内,给予药物或 RSV(200 毫克/千克/天,口服)。另加两个对照组[STZ 处理组(No-ITx-Control)和 STZ+RSV 处理组(No-ITx-RSV)]。ITx后14天进行葡萄糖耐量试验(GTT)。在体外,用药物或 RSV(1 μM)预处理的离体胰岛在缺氧室(O2 1%,1 小时)中孵育。部分 ITx 在小鼠胰岛素 1 基因启动子-绿色荧光蛋白(MIP-GFP)转基因小鼠体内进行,并使用体内成像系统进行分析。经过 14 天的 ITx 治疗后,RSV 治疗组的 GTT 2 小时血糖水平明显低于其他对照组。但 RSV 无 ITx 小鼠的血糖状况并未得到改善。第 3 天,RSV-ITx 组小鼠胰岛移植物中 Ki-67/ 胰岛素共染细胞的百分比明显增加。用抗胰岛素抗体和抗BS-1抗体进行免疫染色显示,RSV处理的胰岛移植物中胰岛素染色面积和血管密度明显增加。通过体内成像测量的每个胰岛移植物的平均血管体积在第 3 天时明显高于 RSV 处理组。在缺氧条件下培养的离体胰岛中,RSV 预处理可明显降低细胞死亡率和氧化应激。对离体胰岛进行缺氧处理会降低 SIRT-1 mRNA 的表达,而 RSV 预处理可恢复这种降低。我们的数据表明,RSV 治疗可改善糖尿病小鼠的血糖控制、β 细胞增殖、降低氧化应激、增强胰岛血管再通以及 ITx 的疗效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Effect of resveratrol treatment on graft revascularization after islet transplantation in streptozotocin-induced diabetic mice.

We evaluated the effect of resveratrol (RSV) on graft survival after islet transplantation (ITx) in diabetic mice. Isolated islets from Balb/c mice (200 IEQ) were transplanted under the kidney capsule of diabetic Balb/c mice. Vehicle or RSV (200 mg/kg/day, orally) was given for 14 days after ITx. Two more control groups [STZ-treated (No-ITx-Control) and STZ+RSV-treated (No-ITx-RSV) mice without ITx] were added. Glucose tolerance tests (GTT) was performed at 14 days after ITx. In vitro, isolated islets pretreated with vehicle or RSV (1 μM) were incubated in a hypoxic chamber (O2 1%, 1hr). Some of the ITx was performed in mouse insulin 1 gene promoter-green fluorescent protein (MIP-GFP) transgenic mice and analyzed using an in vivo imaging system. After 14 days of ITx, 2-hr glucose levels on GTT in the RSV-treated group were significantly lower than those of other control groups. But the glucose status was not improved in No-ITx mice with RSV. At day 3, the percentage of Ki-67/insulin co-stained cells in islet graft was significantly increased in the RSV-ITx group. Immunostaining with anti-insulin and anti-BS-1 antibodies revealed significantly higher insulin-stained area and vascular density in RSV-treated islet grafts. The mean vessel volume per islet graft measured by in vivo imaging was significantly higher in the RSV-treated group at day 3. In isolated islets cultured in hypoxic conditions, the cell death rate and oxidative stress were significantly attenuated with RSV pretreatment. Hypoxic treatment for isolated islets decreased the expression of SIRT-1 mRNA, and this attenuation was recovered by RSV pretreatment. Our data suggest that RSV treatment improved glycemic control, beta-cell proliferation, reduced oxidative stress, and enhanced islet revascularization and the outcome of ITx in diabetic mice.

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来源期刊
Islets
Islets ENDOCRINOLOGY & METABOLISM-
CiteScore
3.30
自引率
4.50%
发文量
10
审稿时长
>12 weeks
期刊介绍: Islets is the first international, peer-reviewed research journal dedicated to islet biology. Islets publishes high-quality clinical and experimental research into the physiology and pathology of the islets of Langerhans. In addition to original research manuscripts, Islets is the leading source for cutting-edge Perspectives, Reviews and Commentaries. Our goal is to foster communication and a rapid exchange of information through timely publication of important results using print as well as electronic formats.
期刊最新文献
3D evaluation of the extracellular matrix of hypoxic pancreatic islets using light sheet fluorescence microscopy. Serum from pregnant donors induces human beta cell proliferation. Characterizing the effects of Dechlorane Plus on β-cells: a comparative study across models and species. Decreased islet amyloid polypeptide staining in the islets of insulinoma patients. Human research islet cell culture outcomes at the Alberta Diabetes Institute IsletCore.
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