{"title":"改进的HPLC-TOF/MS联用在线dph - hplc法对木瓜果实中抗氧化剂的化学性质及评价","authors":"Minmin Zhang, Ruixuan Zhao, Siduo Zhou, Wei Liu, Yan Liang, Zhiguo Zhao, Shengbo Li, Xiao Wang, Tityee Wong, Hengqiang Zhao","doi":"10.1080/10590501.2017.1418814","DOIUrl":null,"url":null,"abstract":"<p><p>An improved method based on HPLC-TOF/MS was developed to catalog the antioxidants in five species of Chaenomeles (Mugua). Forty-four fractions from the Mugua extracts show appreciable levels of antioxidative activity in scavenging the stable free-radical 2,2-diphenyl-1-picrylhydrazyl and the hydroxyl radicals. Twelve major antioxidant's chemical structures are identified. Antioxidant activities differ between species, but intra-species level of antioxidants, regardless of their ripeness, are similar. C. sinensis has the highest antioxidant level. A rigorous quality control procedure was implemented to ensure accuracy of antioxidant quantification. This improved procedure can be used for rapid discovery of antioxidants in other plant extracts.</p>","PeriodicalId":51085,"journal":{"name":"Journal of Environmental Science and Health Part C-Environmental Carcinogenesis & Ecotoxicology Reviews","volume":"36 1","pages":"43-62"},"PeriodicalIF":0.0000,"publicationDate":"2018-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10590501.2017.1418814","citationCount":"15","resultStr":"{\"title\":\"Chemical characterization and evaluation of the antioxidants in Chaenomeles fruits by an improved HPLC-TOF/MS coupled to an on-line DPPH-HPLC method.\",\"authors\":\"Minmin Zhang, Ruixuan Zhao, Siduo Zhou, Wei Liu, Yan Liang, Zhiguo Zhao, Shengbo Li, Xiao Wang, Tityee Wong, Hengqiang Zhao\",\"doi\":\"10.1080/10590501.2017.1418814\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>An improved method based on HPLC-TOF/MS was developed to catalog the antioxidants in five species of Chaenomeles (Mugua). Forty-four fractions from the Mugua extracts show appreciable levels of antioxidative activity in scavenging the stable free-radical 2,2-diphenyl-1-picrylhydrazyl and the hydroxyl radicals. Twelve major antioxidant's chemical structures are identified. Antioxidant activities differ between species, but intra-species level of antioxidants, regardless of their ripeness, are similar. C. sinensis has the highest antioxidant level. A rigorous quality control procedure was implemented to ensure accuracy of antioxidant quantification. This improved procedure can be used for rapid discovery of antioxidants in other plant extracts.</p>\",\"PeriodicalId\":51085,\"journal\":{\"name\":\"Journal of Environmental Science and Health Part C-Environmental Carcinogenesis & Ecotoxicology Reviews\",\"volume\":\"36 1\",\"pages\":\"43-62\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-01-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1080/10590501.2017.1418814\",\"citationCount\":\"15\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Environmental Science and Health Part C-Environmental Carcinogenesis & Ecotoxicology Reviews\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/10590501.2017.1418814\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2018/1/16 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Environmental Science and Health Part C-Environmental Carcinogenesis & Ecotoxicology Reviews","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/10590501.2017.1418814","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2018/1/16 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
Chemical characterization and evaluation of the antioxidants in Chaenomeles fruits by an improved HPLC-TOF/MS coupled to an on-line DPPH-HPLC method.
An improved method based on HPLC-TOF/MS was developed to catalog the antioxidants in five species of Chaenomeles (Mugua). Forty-four fractions from the Mugua extracts show appreciable levels of antioxidative activity in scavenging the stable free-radical 2,2-diphenyl-1-picrylhydrazyl and the hydroxyl radicals. Twelve major antioxidant's chemical structures are identified. Antioxidant activities differ between species, but intra-species level of antioxidants, regardless of their ripeness, are similar. C. sinensis has the highest antioxidant level. A rigorous quality control procedure was implemented to ensure accuracy of antioxidant quantification. This improved procedure can be used for rapid discovery of antioxidants in other plant extracts.
期刊介绍：
Journal of Environmental Science and Health, Part C: Environmental Carcinogenesis and Ecotoxicology Reviews aims at rapid publication of reviews on important subjects in various areas of environmental toxicology, health and carcinogenesis. Among the subjects covered are risk assessments of chemicals including nanomaterials and physical agents of environmental significance, harmful organisms found in the environment and toxic agents they produce, and food and drugs as environmental factors. It includes basic research, methodology, host susceptibility, mechanistic studies, theoretical modeling, environmental and geotechnical engineering, and environmental protection. Submission to this journal is primarily on an invitational basis. All submissions should be made through the Editorial Manager site, and are subject to peer review by independent, anonymous expert referees. Please review the instructions for authors for manuscript submission guidance.
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