[hevin -like抗菌肽WAMPs的基因编码:对植物激素和环境因素的表达]。

Genetika Pub Date : 2016-11-01
E A Istomina, T V Korostyleva, N A Rozhnova, E A Rogozhin, V A Pukhalskiy, T I Odintsova
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引用次数: 0

摘要

我们研究了WAMP家族的hevin -like抗菌肽基因在保护小麦植株免受生物和非生物胁迫中的作用。采用半定量RT-PCR方法,研究了小麦幼苗wamp基因在病原菌侵染、植物激素和重金属离子镉胁迫下的表达。我们发现,小麦胚芽受到有害真菌污染后,wamp家族基因的表达显著增加,主要转录物为wamp-2。我们确定水杨酸,而不是茉莉酸甲酯,诱导wamp家族基因的表达。研究表明,镉离子诱导的非生物胁迫抑制了根中wamp基因的表达,但对其在茎中的表达没有影响。这些结果支持了wamp基因在小麦植物对病原体感染的反应中的保护作用。反过来,对镉离子诱导的非生物胁迫的抗性似乎与wamp家族基因的表达无关。
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[Genes encoding hevein-like antimicrobial peptides WAMPs: Expression in response to phytohormones and environmental factors].

We investigated the role of genes of hevein-like antimicrobial peptides of the WAMP family in the protection of wheat plants against biotic and abiotic stress. The semiquantitative RT-PCR method was used to examine the expression of wamp genes in wheat seedlings in response to infection by pathogens and exposure to phytohormones and ions of a heavy metal ion—cadmium. We discovered that wheat germ contamination by harmful fungi significantly increases expression of genes of the wamp family, and the primary transcript is wamp-2. We determined that salicylic acid, rather than methyl jasmonate, induces expression of genes of the wamp family. We showed that abiotic stress induced by cadmium ions inhibits expression of wamp genes in the roots with no effect on their expression in shoots. The results support the protective role of wamp genes in the response of wheat plants to infections by pathogens. In turn, the resistance to abiotic stress induced by cadmium ions does not appear to be associated with expression of genes of the wamp family.

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