乳发酵短杆菌隐质粒pBL90的核苷酸序列及结构分析

Genetika Pub Date : 2016-11-01
A D Novikov, L E Ryabchenko, T E Shustikova, A V Beletsky, A V Mardanov, N V Ravin, A S Yanenko
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引用次数: 0

摘要

测定了乳发酵短杆菌DSM 1412细胞中检测到的隐质粒pBL90的核苷酸序列。质粒DNA长度为67826 bp。pBL90的核苷酸序列与已知的质粒序列比较,没有明显的同源长区。质粒DNA的计算机分析显示有29个开放阅读框(orf)。15个orf(约占质粒长度的25%)的氨基酸序列与棒状杆菌代表的不同质粒的蛋白质(包括复制蛋白)具有很高(>70%)的同源性。在pBL90中,不同寻常的是存在来自两个不同家族和复制类型的复制基因。
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[Nucleotide sequence and structural analysis of cryptic plasmid pBL90 from Brevibacterium lactofermentum].

The nucleotide sequence of cryptic plasmid (designated as pBL90) detected in the cells of Brevibacterium lactofermentum DSM 1412 was determined. The length of plasmid DNA is 67826 bp. Comparison of the nucleotide sequence of pBL90 with known plasmid sequences showed no long regions of significant homology. Computer analysis of the plasmid DNA revealed 29 open reading frames (ORFs). The amino acid sequences of 15 ORFs (approximately 25% of plasmid length) have a high (>70%) level of identity to proteins from different plasmids of Corynebacterium representatives, including replicative proteins. Unusual in pBL90 is the presence of replicative genes from two different families and types of replication.

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