综合筛选结果表明,麦角硫因是野生大型真菌根瘤菌中含量最丰富的抗氧化剂。

Hengqiang Zhao, Minmin Zhang, Qian Liu, Xiaoli Wang, Ruixuan Zhao, Yanling Geng, Tityee Wong, Shengbo Li, Xiao Wang
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引用次数: 8

摘要

采用亲水相互作用液相色谱法(HILIC)和反相高效液相色谱法(RP -HPLC)分别对野生蘑菇根瘤的极性和非极性提取物进行分离。分离阀将洗脱液分成两部分,用于自由基清除分析和结构鉴定。46种化合物对稳定自由基2,2-二苯基-1-picrylhydrazyl (DPPH)具有清除活性。采用质谱法对8种抗氧化剂(肌苷、咖啡酸、麦角硫因、对羟基苯甲酸、腺苷、3,4-二羟基苯甲醛、芹菜素和柚皮素)的结构进行了表征。其中麦角硫因含量最高(>65%),抗氧化剂活性最高。
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A comprehensive screening shows that ergothioneine is the most abundant antioxidant in the wild macrofungus Phylloporia ribis Ryvarden.

The polar and non-polar extracts from the authenticated wild mushroom Phylloporia ribis were separated by hydrophilic interaction liquid chromatography (HILIC) and by reverse phase (RP)-HPLC, respectively. A split valve separated the eluents into two fractions for free-radical scavenging analysis and for structural identification. Forty-six compounds showed scavenging activity of the stable-free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). The structures of 8 antioxidants (inosine, caffeic acid, ergothioneine, p-hydroxybenzoic acid, adenosine, 3,4-dihydroxybenzaldehyde, apigenin, and naringenin) are characterized by Mass Spectrometer. Among them, ergothioneine was the most abundant (>65%) and most active antioxidant in P. ribis.

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来源期刊
CiteScore
6.20
自引率
0.00%
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审稿时长
>24 weeks
期刊介绍: Journal of Environmental Science and Health, Part C: Environmental Carcinogenesis and Ecotoxicology Reviews aims at rapid publication of reviews on important subjects in various areas of environmental toxicology, health and carcinogenesis. Among the subjects covered are risk assessments of chemicals including nanomaterials and physical agents of environmental significance, harmful organisms found in the environment and toxic agents they produce, and food and drugs as environmental factors. It includes basic research, methodology, host susceptibility, mechanistic studies, theoretical modeling, environmental and geotechnical engineering, and environmental protection. Submission to this journal is primarily on an invitational basis. All submissions should be made through the Editorial Manager site, and are subject to peer review by independent, anonymous expert referees. Please review the instructions for authors for manuscript submission guidance.
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