{"title":"对环带动物 Capitella teleta 后部再生细胞起源的研究。","authors":"Danielle M de Jong, Elaine C Seaver","doi":"10.1002/reg2.94","DOIUrl":null,"url":null,"abstract":"<p><p>Many animals can regenerate, although there is great diversity in regenerative capabilities. A major question in regenerative biology is determining the cellular source of newly formed tissue. The polychaete annelid, <i>Capitella teleta</i>, can regenerate posterior segments following transverse amputation. However, the source, behavior and molecular characteristics of the cells that form new tissue during regeneration are largely unknown. Using an indirect cell tracking method involving 5'-ethynyl-2'-deoxyuridine (EdU) incorporation, we show that cell migration occurs during <i>C. teleta</i> posterior regeneration. Expression of the multipotency/germ line marker <i>CapI-vasa</i> led us to hypothesize that stem cells originate from a multipotent progenitor cell (MPC) cluster, migrate through the coelomic cavity, and contribute to regeneration of tissue. We show that the capacity for posterior regeneration and segment formation is greater with than without the MPC cluster. Finally, we propose a working model of posterior regeneration in <i>C. teleta</i>. This work is the first in <i>C. teleta</i> that addresses the potential source of cells contributing to posterior regeneration, and may provide clues as to why some animals are highly successful regenerators.</p>","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5911572/pdf/","citationCount":"0","resultStr":"{\"title\":\"Investigation into the cellular origins of posterior regeneration in the annelid <i>Capitella teleta</i>.\",\"authors\":\"Danielle M de Jong, Elaine C Seaver\",\"doi\":\"10.1002/reg2.94\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Many animals can regenerate, although there is great diversity in regenerative capabilities. A major question in regenerative biology is determining the cellular source of newly formed tissue. The polychaete annelid, <i>Capitella teleta</i>, can regenerate posterior segments following transverse amputation. However, the source, behavior and molecular characteristics of the cells that form new tissue during regeneration are largely unknown. Using an indirect cell tracking method involving 5'-ethynyl-2'-deoxyuridine (EdU) incorporation, we show that cell migration occurs during <i>C. teleta</i> posterior regeneration. Expression of the multipotency/germ line marker <i>CapI-vasa</i> led us to hypothesize that stem cells originate from a multipotent progenitor cell (MPC) cluster, migrate through the coelomic cavity, and contribute to regeneration of tissue. We show that the capacity for posterior regeneration and segment formation is greater with than without the MPC cluster. Finally, we propose a working model of posterior regeneration in <i>C. teleta</i>. This work is the first in <i>C. teleta</i> that addresses the potential source of cells contributing to posterior regeneration, and may provide clues as to why some animals are highly successful regenerators.</p>\",\"PeriodicalId\":90316,\"journal\":{\"name\":\"Regeneration (Oxford, England)\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-12-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5911572/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Regeneration (Oxford, England)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/reg2.94\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2018/3/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Regeneration (Oxford, England)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/reg2.94","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2018/3/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
尽管再生能力存在很大差异,但许多动物都能再生。再生生物学的一个主要问题是确定新形成组织的细胞来源。多毛环节动物 Capitella teleta 可以在横向截肢后再生后节。然而,在再生过程中形成新组织的细胞的来源、行为和分子特征在很大程度上是未知的。我们利用 5'-乙炔基-2'-脱氧尿苷(EdU)掺入的间接细胞追踪方法,证明了 C. teleta 后节再生过程中会发生细胞迁移。多能/胚系标记 CapI-vasa 的表达使我们推测,干细胞起源于多能祖细胞(MPC)群,通过腹腔迁移,促进组织再生。我们的研究表明,有多潜能祖细胞集群比没有多潜能祖细胞集群的后部再生和节段形成能力更强。最后,我们提出了 C. teleta 后部再生的工作模型。这项工作是首次在 C. teleta 中研究有助于后部再生的细胞的潜在来源,并可能为为什么某些动物是非常成功的再生者提供线索。
Investigation into the cellular origins of posterior regeneration in the annelid Capitella teleta.
Many animals can regenerate, although there is great diversity in regenerative capabilities. A major question in regenerative biology is determining the cellular source of newly formed tissue. The polychaete annelid, Capitella teleta, can regenerate posterior segments following transverse amputation. However, the source, behavior and molecular characteristics of the cells that form new tissue during regeneration are largely unknown. Using an indirect cell tracking method involving 5'-ethynyl-2'-deoxyuridine (EdU) incorporation, we show that cell migration occurs during C. teleta posterior regeneration. Expression of the multipotency/germ line marker CapI-vasa led us to hypothesize that stem cells originate from a multipotent progenitor cell (MPC) cluster, migrate through the coelomic cavity, and contribute to regeneration of tissue. We show that the capacity for posterior regeneration and segment formation is greater with than without the MPC cluster. Finally, we propose a working model of posterior regeneration in C. teleta. This work is the first in C. teleta that addresses the potential source of cells contributing to posterior regeneration, and may provide clues as to why some animals are highly successful regenerators.