[基于比较代谢组学的合理饲养策略提高阿维菌素产量]。

微生物学报 Pub Date : 2017-02-04
Peng Cao, Dong Hu, Jun Zhang, Bianqiang Zhang, Qiang Gao
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引用次数: 0

摘要

目的:为了揭示阿维菌素的关键代谢物和代谢途径,采用比较代谢组学方法分析阿维菌素链霉菌在不同发酵培养基中细胞内关键代谢物的差异。采用合理饲养法提高阿维菌素产量。[方法]采用基于GC-MS的比较细胞内代谢组学方法对M1和M2培养基中的菌丝样品进行分析。针对相关代谢途径的不足,分别进行了单一前体补充和联合前体补充,获得了提高阿维菌素产量的优化培养基M3。[结果]共鉴定出232种细胞内代谢物,其中70种准确匹配,最终通过PCA和PLS分析验证了21种影响阿维菌素生物合成的代谢物。其中乳酸、丙酮酸、琥珀酸、苏氨酸、异亮氨酸、缬氨酸和脂质对阿维菌素滴度的影响较为明显。当在不同时间点将这些前体一起供给M2时,滴度从5.36 g/L提高到5.92 g/L,提高了10.4%。结论:通过比较代谢组学分析和合理优化饲养,阿维菌素产量明显增加。为提高生物制品的产量提供了一条新的途径。
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[Enhanced avermectin production by rational feeding strategies based on comparative metabolomics].

Objective: In order to reveal key metabolites and metabolic pathways of avermectin, comparative metabolomics approach was used to analyze the difference of key intracellular metabolites of Streptomyces avermitilis in different fermentation media. Then the rational feeding approach was used to enhance avermectin production.[

Methods: Mycelial samples in M1 and M2 media were analyzed by GC-MS based comparative intracellular metabolomics. Based on the deficiency of relative metabolic pathways, single precursor supplement and combined precursors supplement were exerted, and the optimized medium M3 was obtained to enhance avermectin production.[

Results: In total 232 intracellular metabolites were identified and 70 of them were accurately matched, and 21 metabolites influencing avermectin biosynthesis were finally verified by PCA and PLS analyses. Among them, lactic acid, pyruvic acid, succinic acid, threonine, isoleucine, valine and lipids determined the avermectin titer more obviously. When supplying with these precursors together to M2 at different time points, the titer increased by 10.4% from 5.36 g/L to 5.92 g/L.

Conclusion: The production of avermectin increased apparently with the help of comparative metabolomics analysis and rational feeding optimization. It also provides a new approach to enhance the yield of bioproducts.

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来源期刊
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期刊介绍: Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.
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