水产养殖水净化乳酸菌的分离鉴定及发酵优化

微生物学报 Pub Date : 2017-02-04
Fengxing Xie, Fengfeng Zhang, Ke Zhou, Yujie Zhao, Qiong Zhao, Haibo Sun
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引用次数: 0

摘要

目的:从养殖环境和对虾肠道中筛选乳酸菌,以获得用于水产养殖水净化的优良菌种。方法:对模拟废水中乳酸菌在常温和低温下的潜在净水能力进行评价。采用形态生理生化特征、16S rRNA基因序列分析对菌株r13进行鉴定。采用单因素试验和正交设计试验对r13发酵进行优化。结果:从3份样品中共分离到136株乳酸菌。净水试验结果表明,r13对水中的亚硝酸盐和氨有较高的去除能力。r13处理72 h后,水中浓度为11.5 mg/L的亚硝酸盐被完全去除,当初始浓度为13.0 mg/L时,氨的降解率为29.1%。根据形态学、生理生化特征及16S rRNA基因序列分析,r13为植物乳杆菌。r13的最佳发酵条件为:酵母浸膏6.0 g/L、葡萄糖20.0 g/L、乙酸钠4.0 g/L、柠檬酸氢二钠2.0 g/L、磷酸二钾2.0 g/L、番茄汁50 mL,接种量5% (V/V), pH 6.0, 34℃。在此条件下培养72 h,细菌生物量达到28.4 g/L湿重,细胞计数达到4.4×109 CFU/mL。结论:r13具有良好的亚硝酸盐去除能力,是一种很有前景的水产养殖水体净化微生物。
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[Isolation, identification and fermentation optimization of lactic acid bacteria for aquaculture water purification].

Objective: In order to get excellent strains for aquiculture water purification, we screened lactic acid bacteria from the aquaculture environment and intestinal tract of shrimp.

Methods: The potential water purification ability of lactic acid bacteria at normal and low temperature was evaluated in the simulated wastewater. Morphological physio-biochemical characteristics, 16S rRNA gene sequence analysis were used to identify strain r13. Single factor test and orthogonal-design experiment were applied to optimize fermentation for r13.

Results: In total 136 lactic acid bacteria strains were isolated from 3 samples. The results of water purification test suggested r13 had higher removal ability of nitrite and ammonia from water. After 72 h treatment by r13, nitrite with 11.5 mg/L in the water was completely removed and ammonia degradation rate was 29.1% with 13.0 mg/L original concentration. According to morphological, physio-biochemical characteristics and 16S rRNA gene sequence analysis, r13 was identified as Lactobacillus plantarum. The optimal fermentation condition for r13 was 6.0 g/L yeast extract, 20.0 g/L glucose, 4.0 g/L sodium acetate, 2.0 g/L diammonium hydrogen citrate, 2.0 g/L monopotassium phosphate, 50 mL tomato juice, with inoculation rate 5% (V/V), at pH 6.0 and 34℃. Under this condition cultured for 72 h, the bacterial biomass reached 28.4 g/L wet weight and cell counting reached 4.4×109 CFU/mL.

Conclusion: Considering high nitrite removal ability, we suggested that r13 would be promising microorganism for water purification in aquiculture.

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期刊介绍: Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.
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