{"title":"[DNA核酸酶与猪链球菌血清9型毒力的关系]。","authors":"Tang Huanyu, Haiyan Ren, Zongfu Wu, Chengping Lu","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>In addition to Streptococcus suis serotype 2, Streptococcus suis serotype 9 (SS9) is also a currently prevalent serotype and a zoonotic pathogen. In our previous study, SS9 DNA nuclease (SsnA) was considered as a candidate virulence factor. To clarify the impact of SsnA on SS9 virulence, we constructed ssnA mutant (ΔssnA) and studied its biological functions.</p><p><strong>Methods: </strong>We evaluated the virulence of wild type strain and ΔssnA in a zebrafish infection model and compared the adherence rate to HEp-2 cells, the survival rate in pig blood, and enzymatic activity between wild type stain and ΔssnA.</p><p><strong>Results: </strong>In a zebrafish infection experiment, the 50% lethal dose value of ΔssnA was 11.2-fold higher than that of wild type strain. The adherence rate of ΔssnA to HEp-2 cells was only 60.61% of the wild strain level. The survival rate of ΔssnA in pig blood was declined to 71.88% of wild strain level. The enzymatic activity assay showed that SsnA can degrade both linear and circular DNA.</p><p><strong>Conclusion: </strong>SsnA contributes to SS9 virulence in a zebrafish infection model, the adherence to HEp-2 cells, and the survival in pig blood. SsnA is indeed an essential virulence factor for SS9.</p>","PeriodicalId":7120,"journal":{"name":"微生物学报","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Relationship between DNA nuclease and the virulence of Streptococcus suis serotype 9].\",\"authors\":\"Tang Huanyu, Haiyan Ren, Zongfu Wu, Chengping Lu\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>In addition to Streptococcus suis serotype 2, Streptococcus suis serotype 9 (SS9) is also a currently prevalent serotype and a zoonotic pathogen. In our previous study, SS9 DNA nuclease (SsnA) was considered as a candidate virulence factor. To clarify the impact of SsnA on SS9 virulence, we constructed ssnA mutant (ΔssnA) and studied its biological functions.</p><p><strong>Methods: </strong>We evaluated the virulence of wild type strain and ΔssnA in a zebrafish infection model and compared the adherence rate to HEp-2 cells, the survival rate in pig blood, and enzymatic activity between wild type stain and ΔssnA.</p><p><strong>Results: </strong>In a zebrafish infection experiment, the 50% lethal dose value of ΔssnA was 11.2-fold higher than that of wild type strain. The adherence rate of ΔssnA to HEp-2 cells was only 60.61% of the wild strain level. The survival rate of ΔssnA in pig blood was declined to 71.88% of wild strain level. The enzymatic activity assay showed that SsnA can degrade both linear and circular DNA.</p><p><strong>Conclusion: </strong>SsnA contributes to SS9 virulence in a zebrafish infection model, the adherence to HEp-2 cells, and the survival in pig blood. SsnA is indeed an essential virulence factor for SS9.</p>\",\"PeriodicalId\":7120,\"journal\":{\"name\":\"微生物学报\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-04-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"微生物学报\",\"FirstCategoryId\":\"1089\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"微生物学报","FirstCategoryId":"1089","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Relationship between DNA nuclease and the virulence of Streptococcus suis serotype 9].
Objective: In addition to Streptococcus suis serotype 2, Streptococcus suis serotype 9 (SS9) is also a currently prevalent serotype and a zoonotic pathogen. In our previous study, SS9 DNA nuclease (SsnA) was considered as a candidate virulence factor. To clarify the impact of SsnA on SS9 virulence, we constructed ssnA mutant (ΔssnA) and studied its biological functions.
Methods: We evaluated the virulence of wild type strain and ΔssnA in a zebrafish infection model and compared the adherence rate to HEp-2 cells, the survival rate in pig blood, and enzymatic activity between wild type stain and ΔssnA.
Results: In a zebrafish infection experiment, the 50% lethal dose value of ΔssnA was 11.2-fold higher than that of wild type strain. The adherence rate of ΔssnA to HEp-2 cells was only 60.61% of the wild strain level. The survival rate of ΔssnA in pig blood was declined to 71.88% of wild strain level. The enzymatic activity assay showed that SsnA can degrade both linear and circular DNA.
Conclusion: SsnA contributes to SS9 virulence in a zebrafish infection model, the adherence to HEp-2 cells, and the survival in pig blood. SsnA is indeed an essential virulence factor for SS9.
期刊介绍：
Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal
publishes original papers,reviews in microbiological science,and short communications describing unusual observations.
Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database
(CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical
Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.
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