[通过全基因组关联分析确定大肠杆菌和金黄色葡萄球菌之间的相互作用]。

微生物学报 Pub Date : 2017-04-04
Nan Chen, Jing Zhu, Meixia Ye, Yi Jin, Xiaoqing He, Rongling Wu
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引用次数: 0

摘要

目的:研究两种细菌共培养过程中的相互作用。方法:通过36株大肠杆菌和36株金黄色葡萄球菌的两两共培养,在相互作用环境中监测各菌种的生长情况。我们通过全基因组测序鉴定了许多单核苷酸多态性(SNPs),这些snp被用作预测表型性状变异的遗传标记。采用全基因组关联研究(GWAS)鉴定控制两种间竞争的位点。结果:在大肠杆菌中,影响最大生长率变化的显著snp有162个,影响最大生长率变化的显著snp有36个,影响最大生长率变化的显著snp有36个。我们在这次注释后鉴定出的5个重要的大肠杆菌基因在其他进化研究中也有报道。通过比较初始菌株和单一培养菌株,我们还发现了85个影响金黄色葡萄球菌最大生长速率变化的显著snp。关于细菌数量的变化,我们发现大肠杆菌中有706个显著snp,金黄色葡萄球菌中有129个显著snp。结论:我们在单培养和共培养中都发现了一些影响大肠杆菌与金黄色葡萄球菌相互作用的重要基因。GWAS具有研究细菌种间相互作用的潜力。
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[Interactions between Escherichia coli and Staphylococcus aureus determined by genome-wide association analysis].

Objective: We studied the interactions in a co-culture of two bacteria.

Methods: By pairwise co-culturing of 36 Escherichia coli and 36 Staphylococcus aureus strains, we monitored the growth of each species in an interaction environment. We identified numerous Single Nucleotide Polymorphisms (SNPs) by whole-genome sequencing used as genetic markers to predict variations in phenotypic traits. Genome-wide association study (GWAS) was applied to identify loci that controlled competition between the two species.

Results: In E. coli, 162 significant SNPs affected the change of maximum growth rate by comparing initials strains with those grown in co-culture, and 36 significant SNPs affected the change of maximum growth rate comparing monoculture and co-culture strains. Five of the significant E. coli genes we identified after annotation this time were also reported in other evolutionary studies. We also identified 85 significant SNPs in S. aureus that affected the change of maximum growth rate by comparing initial strains with those grown in monoculture. About the change of bacterial numbers, we found that 706 significant SNPs were associated in E. coli and 129 in S. aureus. Thirteen of the E. coli significant genes in this study were also verified in previous evolutionary reports

Conclusion: We found several significant genes both in monoculture and co-culture affecting the interaction of E. coli and S. aureus. GWAS has the potential to study interspecific interactions of bacteria.

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来源期刊
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期刊介绍: Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.
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