Alice Di Pierro , Heather Bondi , Chiara Monti , Luisa Pieroni , Enrico Cilio , Andrea Urbani , Tiziana Alberio , Mauro Fasano , Maurizio Ronci
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Experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics
Mitochondria possess a proteolytic system that contributes to the regulation of mitochondrial dynamics, mitochondrial biogenesis and mitophagy. We aimed at the identification by bottom-up proteomics of altered protein processing due to the activation of mitochondrial proteases in a cellular model of impaired dopamine homeostasis. Moreover, we optimized the conditions for top-down proteomics to identify the cleavage site sequences.
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