用x射线测定RNA的时间分辨羟基自由基足迹

Q4 Chemistry Current Protocols in Nucleic Acid Chemistry Pub Date : 2018-06-07 DOI:10.1002/cpnc.52

Yumeng Hao, Jen Bohon, Ryan Hulscher, Mollie C. Rappé, Sayan Gupta, Tadepalli Adilakshmi, Sarah A. Woodson

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Time-Resolved Hydroxyl Radical Footprinting of RNA with X-Rays

RNA footprinting by hydroxyl radical cleavage provides ‘snapshots’ of RNA tertiary structure or protein interactions that bury the RNA backbone. Generation of hydroxyl radicals with a high-flux synchrotron X-ray beam provides analysis on a short timescale (5–100 msec), which enables the structures of folding intermediates or other transient conformational states to be determined in biochemical solutions or cells. This article provides protocols for using synchrotron beamlines for hydroxyl radical footprinting. © 2018 by John Wiley & Sons, Inc.

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Current Protocols in Nucleic Acid Chemistry Chemistry-Organic Chemistry

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期刊介绍： Published in association with International Society for Nucleosides, Nucleotides & Nucleic Acids (IS3NA) , Current Protocols in Nucleic Acid Chemistry is equally valuable for biotech, pharmaceutical, and academic labs. It is the resource for designing and running successful research projects in the rapidly growing and changing field of nucleic acid, nucleotide, and nucleoside research.

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