抗β2-糖蛋白I抗体对绒毛膜癌和原代滋养细胞toll样受体mRNA表达的影响。

IF 0.2 4区医学 Q4 Medicine 生殖医学杂志 Pub Date : 2016-09-01

Akikazu Nakamura, Tatsuo Yamamoto, Fumihisa Chishima

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引用次数: 0

摘要

目的:通过观察抗β2- gpi抗体对绒毛膜癌细胞和原代滋养细胞中toll样受体(TLR) mRNA表达的影响，探讨抗β2- gpi抗体对滋养细胞的影响。研究设计:我们用抗β2- gpi抗体阳性和阴性血清中提取的igg培养绒毛膜癌细胞(BeWo)和早期妊娠滋养母细胞。四小时后，我们从这些细胞中纯化rna。我们实时检测细胞中TLR mRNA的表达。PCR。结果:用抗β2- gpi抗体阳性妇女的igg培养的BeWo细胞和原代滋养细胞中，TLR mRNA的表达增加。其中，TLR1、2、4在BeWo细胞中的表达和TLRI、3、4、9在妊娠早期滋养细胞中的表达显著增加。结论:抗β2- gpi抗体阳性IgG可增加绒毛膜癌细胞和原代滋养细胞中TElR mRNA的表达。我们认为抗β2- gpi抗体可能与滋养细胞结合并增加TLR mRNA的表达。

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Effects of Anti-β2-Glycoprotein I Antibody on the Expression of Toll-like Receptors mRNA in Choriocarcinoma and Primary Trophoblast Cells.

Objective: To investigate the effects of the anti- β2-glycoprotein I (anti-β2-GPI) antibody on the tro- phoblast by evaluating the effects of the anti-β2-GPI antibody on the expression of toll-like receptor (TLR) mRNA in choriocarcinoma cells and primary trophoblast cells.

Study design: We cul- tured the choriocarcinoma cells (BeWo) and the pri- mary first trimester tropho- blast with the IgGs taken from anti-β2-GPI antibody-positive and -negative sera. Four hours later we purified the RNAs from those cells. We measured the expressions of TLR mRNA in cells using real-time. PCR.

Results: The expression of TLR mRNA increased in BeWo cells and primary trophoblast cells cultured with the IgGs taken from anti-β2-GPI antibody-positive women. Specifically, the expression of TLR1, 2, and 4 in BeWo cells -and TLRI, 3, 4, and 9 in first trimester trophoblast cells increased significantly.

Conclusion: The anti-β2-GPI antibody-positive IgG increased the TElR mRNA expression in choriocarcino- ma cells and primary trophoblast cells. We suggest that anti-β2-GPI antibodies may bind to trophoblast and increase the expression of TLR mRNA.

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来源期刊

生殖医学杂志医学-妇产科学

自引率

0.00%

发文量

6427

审稿时长

6-12 weeks

期刊介绍： The Journal of Reproductive Medicine® has been the essential tool of Obstetricians and Gynecologists since 1968. As a highly regarded professional journal and the official periodical of six medical associations, JRM® brings timely and relevant information on the latest procedures and advances in the field of reproductive medicine. Published bimonthly, JRM® contains peer-reviewed articles and case reports submitted by top specialists. Common topics include research, clinical practice, and case reports related to general obstetrics and gynecology, infertility, female cancers, gynecologic surgery, contraception, and medical education.