大肠杆菌在液体培养基中的生长

Q2 Biochemistry, Genetics and Molecular Biology Current Protocols in Molecular Biology Pub Date : 2018-11-09 DOI:10.1002/cpmb.81

Karen L. Elbing, Roger Brent

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引用次数: 7

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Growth of E. coli in Liquid Medium

We describe the procedure for inoculating overnight (starter) cultures of E. coli from a single colony, along with considerations for growing larger cultures. We also include two methods for monitoring the number of cells per unit volume (density) of liquid cultures using a spectrophotometer and a hemacytometer or “count slide.” © 2018 by John Wiley & Sons, Inc.

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Current Protocols in Molecular Biology Biochemistry, Genetics and Molecular Biology-Molecular Biology

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