HPV感染的全组织宫颈图谱:人类局灶性潜伏HPV感染的分子证据

Anne Hammer , Maurits NC de Koning , Jan Blaakaer , Torben Steiniche , John Doorbar , Heather Griffin , Else Mejlgaard , Hans Svanholm , Wim GV Quint , Patti E. Gravitt
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引用次数: 22

摘要

在这项研究中,我们旨在提供人类HPV潜伏期的分子证据,并讨论进行潜伏期研究的潜在挑战。我们分析了两名接受子宫切除术的妇女的整个宫颈,与宫颈异常无关。分别分为242套和186套,分别使用SPF10-PCR-DEIA-LiPA25系统进行HPV检测。为了确定是否存在转化性感染或生产性感染的证据,我们使用生物标志物E4和P16INK4a对紧挨着hpv阳性切片的载玻片进行染色。在两种服务中均检测到HPV。患者1中1/242组HPV31阳性。患者2中HPV18阳性13/186,HPV53阳性1/186。通过标记物E4和P16INK4a评估,两例患者的感染都是局灶性的,没有转化性或生产性感染的迹象。如果每个区只分析一组,检测感染的概率分别为32.3%和2%。我们的研究结果支持HPV可能能够在人类子宫颈中建立潜伏期的观点;然而,与潜伏性HPV感染相关的风险仍不清楚。
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Whole tissue cervical mapping of HPV infection: Molecular evidence for focal latent HPV infection in humans

In this study, we aimed to provide molecular evidence of HPV latency in humans and discuss potential challenges of conducting studies on latency. We analyzed the entire cervix of two women who underwent hysterectomy unrelated to cervical abnormality. The cervices were sectioned into 242 and 186 sets respectively, and each set was tested separately for HPV using the SPF10-PCR-DEIA-LiPA25 system. To identify whether there was any evidence of transforming or productive infection, we used the biomarkers E4 and P16INK4a to stain slides immediately adjacent to HPV-positive sections. HPV was detected in both cervices. In patient 1, 1/242 sets was positive for HPV31. In patient 2, 13/186 sets were positive for HPV18 and 1/186 was positive for HPV53. The infection was very focal in both patients, and there was no sign of a transforming or productive infection, as evaluated by the markers E4 and P16INK4a. Had we only analyzed one set from each block, the probability of detecting the infection would have been 32.3% and 2%, respectively.Our findings support the idea that HPV may be able to establish latency in the human cervix; however, the risk associated with a latent HPV infection remains unclear.

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来源期刊
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审稿时长
14 weeks
期刊介绍: The official Journal of the International Papillomavirus Society Papillomavirus Research (PVR), the Journal of HPV and other Small DNA Tumor Viruses publishes innovative papers related to all aspects of papillomaviruses and other small DNA tumor viruses. The official journal of the International Papillomavirus Society, PVR is an open access publication that aims to bring together virologists, immunologists, epidemiologists and clinicians working in the booming field of HPV and animal papillomaviruses, polyomaviruses and other small DNA tumor viruses and their associated diseases, in order to foster and facilitate interdisciplinary communication. The journal welcomes original research articles, reviews, short communications, opinion articles and regional update reports on papillomaviruses and other tumor viruses in the following sections: a. Biology of papillomaviruses and related viruses from life cycle to cancer b. Epidemiology etiology and natural history studies c. Natural and induced immunity including vaccine research d. Intervention studies and strategies including i. Clinical studies and trials ii. HPV treatments iii. HPV vaccination programs iv. Diagnostics and screening e. Infection and disease prevention, modeling studies f. Guidelines and public health recommendations g. HPV Studies in special populations Regional and local studies on these viruses.
期刊最新文献
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