肝细胞癌的PET成像:18f -氟丙酸作为18f -氟脱氧葡萄糖的补充放射性示踪剂

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS Molecular Imaging Pub Date : 2019-01-01 DOI:10.1177/1536012118821032

Jing Zhao, Zhanwen Zhang, Dahong Nie, Hui Ma, Gongjun Yuan, Shu Su, Shaoyu Liu, Sheng Liu, Ganghua Tang

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引用次数: 7

摘要

目的:评价18f -氟丙酸(18F-FPA)和18f -氟脱氧葡萄糖(18F-FDG)正电子发射断层扫描(PET)对hcc的临床前成像价值。方法:在体外和体内观察3种HCC细胞系(Hep3B、HepG2和SK-Hep1)对18F-FPA和18F-FDG的摄取模式。通过奥利司他和5-十四环氧基-2-呋喃酸的抑制实验，研究了18F-FPA的摄取机制。对不同肿瘤动物模型进行18F-FPA PET显像，并与18F-FDG进行比较。我们还评估了这些细胞系中葡萄糖转运蛋白-1 (GLUT1)、脂肪酸合成酶(FASN)和基质金属蛋白酶-2 (MMP2)的表达。结果:体外实验表明，两种示踪剂的摄取模式在HCC细胞系中是互补的。奥利司他和5-十四环氧基-2-呋喃酸降低了18F-FPA的摄取。在SK-Hep1和HepG2肿瘤中，18F-FPA的瘤肝比优于18F-FDG (P < 0.05)。然而，在Hep3B肿瘤中，18F-FDG的肿瘤到肝脏的正常化摄取高于18F-FPA (P < 0.01)。FASN在高18F-FPA摄取的细胞系中高表达，而GLUT1在高18F-FDG摄取的细胞系中高表达。18F-FPA摄取与FASN (r = 0.89, P = 0.014)和MMP2 (r = 0.77, P = 0.002)表达相关。结论:PET + 18F-FPA联合18F-FDG可作为肝癌检测的替代方法。

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PET Imaging of Hepatocellular Carcinomas: ¹⁸F-Fluoropropionic Acid as a Complementary Radiotracer for ¹⁸F-Fluorodeoxyglucose.

Objective: To evaluate the preclinical value of ¹⁸F-fluoropropionic acid (¹⁸F-FPA) and ¹⁸F-fluorodeoxyglucose (¹⁸F-FDG) positron emission tomography (PET) for imaging HCCs.

Methods: The ¹⁸F-FPA and ¹⁸F-FDG uptake patterns in 3 HCC cell lines (Hep3B, HepG2, and SK-Hep1) were assessed in vitro and in vivo. The ¹⁸F-FPA uptake mechanism was investigated using inhibition experiments with orlistat and 5-tetradecyloxy-2-furoic acid. The ¹⁸F-FPA PET imaging was performed in different tumor animal models and compared with ¹⁸F-FDG. We also evaluated the expressions of glucose transporter-1 (GLUT1), fatty acid synthase (FASN), and matrix metalloproteinase-2 (MMP2) in these cell lines.

Results: In vitro experiments showed that the radiotracer uptake patterns were complementary in the HCC cell lines. Orlistat and 5-tetradecyloxy-2-furoic acid decreased the uptake of ¹⁸F-FPA. The tumor-to-liver ratio of ¹⁸F-FPA was superior to that of ¹⁸F-FDG in the SK-Hep1 and HepG2 tumors ( P < .05). However, in the Hep3B tumors, the tumor-to-liver normalized uptake of ¹⁸F-FDG was higher than ¹⁸F-FPA ( P < .01). FASN was highly expressed in cell lines with high ¹⁸F-FPA uptake, whereas GLUT1 was highly expressed in cell lines with high ¹⁸F-FDG uptake. The ¹⁸F-FPA uptake correlated with FASN ( r = 0.89, P = .014) and MMP2 ( r = 0.77, P = .002) expressions.

Conclusions: PET imaging with ¹⁸F-FPA combined with ¹⁸F-FDG can be an alternative for detecting HCC.

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Molecular Imaging Biochemistry, Genetics and Molecular Biology-Biotechnology

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3.60%

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期刊介绍： Molecular Imaging is a peer-reviewed, open access journal highlighting the breadth of molecular imaging research from basic science to preclinical studies to human applications. This serves both the scientific and clinical communities by disseminating novel results and concepts relevant to the biological study of normal and disease processes in both basic and translational studies ranging from mice to humans.