来自南非阴茎拭子的六种新型伽玛乳头瘤病毒的发现、特征和基因组变异

Alltalents T. Murahwa , Tracy L. Meiring , Zizipho Z.A. Mbulawa , Anna-Lise Williamson
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引用次数: 10

摘要

从阴茎拭子中鉴定了六种新型人乳头瘤病毒。每个新类型均获得多个全基因组克隆,全基因组大小分别为:HPV211 (7253bp)、HPV212 (7208bp)、HPV213 (7096bp)、HPV214(7357)、HPV215 (7186bp)和HPV216 (7233bp)。新型乳头瘤病毒在系统发育上均与γ乳头瘤病毒聚集;HPV211与γ -8种的HPV168亲缘关系最密切(L1核苷酸序列同源度为72%),HPV212与γ -17种的HPV144亲缘关系最密切(82.9%),HPV213与γ -13种的HPV153亲缘关系最密切(71.8%),HPV214与γ -6种的HPV103亲缘关系最密切(75.3%),HPV215和HPV216与γ -9种的HPV129亲缘关系最密切(分别为76.8%和79.2%)。新的HPV类型显示了经典的gammapapillomavirus的基因组组织,有7个开放阅读框(orf)编码5个早期(E1, E2, E4, E6和E7)和2个晚期(L1和L2)蛋白。作为典型的γ乳头瘤病毒,新型病毒均缺乏E5 ORF, HPV214也缺乏E6 ORF。HPV212有9个独特的变体,HPV213有5个,HPV215有4个变体。新类型的保守结构域包括锌指结合结构域和PDZ结构域。在HPV214中还发现了E7蛋白的一个视网膜母细胞瘤结合域(pRB)结合域。这项研究扩大了快速增长的伽玛乳头瘤病毒属的知识。
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Discovery, characterisation and genomic variation of six novel Gammapapillomavirus types from penile swabs in South Africa

Six novel human papillomaviruses from penile swabs were characterised. Multiple full genome clones for each novel type were generated, and complete genome sizes were: HPV211 (7253bp), HPV212 (7208bp), HPV213 (7096bp), HPV214 (7357), HPV215 (7186bp) and HPV216 (7233bp). Phylogenetically the novel papillomaviruses all clustered with Gammapapillomaviruses: HPV211 is most closely related to HPV168 (72% identity in the L1 nucleotide sequence) of the Gamma-8 species, HPV212 is most closely related to HPV144 (82.9%) of the Gamma-17 species, HPV213 is most closely related to HPV153 (71.8%) of the Gamma-13 species, HPV214 is most closely related to HPV103 (75.3%) of the Gamma-6 species, HPV215 and HPV216 are most closely related to HPV129 (76.8% and 79.2% respectively) of the Gamma-9 species. The novel HPV types demonstrated the classical genomic organisation of Gammapapillomavirusess, with seven open reading frames (ORFs) encoding five early (E1, E2, E4, E6 and E7) and two late (L1 and L2) proteins. Typical of Gammapapillomavirusess the novel types all lacked the E5 ORF and HPV214 also lacked the E6 ORF. HPV212 had nine unique variants, HPV213 had five and HPV215 had four variants. Conserved domains observed among the novel types are the Zinc finger Binding Domain and PDZ domains. A retinoblastoma binding domain (pRB) binding domain in E7 protein was additionally identified in HPV214. This study expands the knowledge of the rapidly growing Gammapapillomavirus genus.

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来源期刊
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审稿时长
14 weeks
期刊介绍: The official Journal of the International Papillomavirus Society Papillomavirus Research (PVR), the Journal of HPV and other Small DNA Tumor Viruses publishes innovative papers related to all aspects of papillomaviruses and other small DNA tumor viruses. The official journal of the International Papillomavirus Society, PVR is an open access publication that aims to bring together virologists, immunologists, epidemiologists and clinicians working in the booming field of HPV and animal papillomaviruses, polyomaviruses and other small DNA tumor viruses and their associated diseases, in order to foster and facilitate interdisciplinary communication. The journal welcomes original research articles, reviews, short communications, opinion articles and regional update reports on papillomaviruses and other tumor viruses in the following sections: a. Biology of papillomaviruses and related viruses from life cycle to cancer b. Epidemiology etiology and natural history studies c. Natural and induced immunity including vaccine research d. Intervention studies and strategies including i. Clinical studies and trials ii. HPV treatments iii. HPV vaccination programs iv. Diagnostics and screening e. Infection and disease prevention, modeling studies f. Guidelines and public health recommendations g. HPV Studies in special populations Regional and local studies on these viruses.
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