{"title":"黄连木果实评估化学诱导记忆损伤小鼠学习记忆潜能的药理学研究。","authors":"Satyam Singh, Dharamveer, Mayank Kulshreshtha","doi":"10.2174/1871524919666190304122927","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>The present study was designed to investigate the potential of Pistacia vera (P. vera) fruits in experimental memory impairments in mice.</p><p><strong>Material & methods: </strong>Memory impairment was induced in Swiss Albino mice by scopolamine (0.4mg mg/kg. i.p). Animals were divided into five separate groups of six animals each, positive control group received carboxy methyl cellulose (CMC) as vehicle, negative control group received scopolamine with vehicle, and standard group received donepezil (5mg/kg i.p) with Scopolamine. Ethanolic extract of P. vera (EEPV) at doses of 200mg/kg & 400mg/kg p.o were administered to group test1 & test 2 respectively along with scopolamine. Elevated plus maze (EPM), passive avoidance paradigms and morris water maze (MWM) were used as exteroceptive behavioral models to access learning and memory activity. Transfer latency, step down latency and escape latency parameters were evaluated plus maze, passive avoidance paradigm, morris water maze. Thereafter lipid peroxidation test, glutathione level and catalase activities were estimated in homogenized brain of mice.</p><p><strong>Results: </strong>Pretreatment of mice with EEPV (200mg/kg & 400mg/kg) significantly reduced scopolamine induced amnesia. The obtained data clearly revealed that there was increase in escape latency in MWM and also increase in step down latency in passive avoidance paradigm. Transfer latencey was found to be decrease in EPM and biochemical. Parameters were clearly satisfied the data as compared to negative control group which was indicative of cognitive improvement.</p><p><strong>Conclusion: </strong>P. vera fruit extract demonstrated to improve cognitive process by enhancing memory in different experimental paradigm such as EPM, passive avoidance and MWM when administered orally. Hence it would be worthwhile to explore the potential of this plant in the management of memory disorders.</p>","PeriodicalId":9799,"journal":{"name":"Central nervous system agents in medicinal chemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":"{\"title\":\"Pharmacological Approach of Pistacia Vera Fruit to Assess Learning and Memory Potential in Chemically-Induced Memory Impairment in Mice.\",\"authors\":\"Satyam Singh, Dharamveer, Mayank Kulshreshtha\",\"doi\":\"10.2174/1871524919666190304122927\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>The present study was designed to investigate the potential of Pistacia vera (P. vera) fruits in experimental memory impairments in mice.</p><p><strong>Material & methods: </strong>Memory impairment was induced in Swiss Albino mice by scopolamine (0.4mg mg/kg. i.p). Animals were divided into five separate groups of six animals each, positive control group received carboxy methyl cellulose (CMC) as vehicle, negative control group received scopolamine with vehicle, and standard group received donepezil (5mg/kg i.p) with Scopolamine. Ethanolic extract of P. vera (EEPV) at doses of 200mg/kg & 400mg/kg p.o were administered to group test1 & test 2 respectively along with scopolamine. Elevated plus maze (EPM), passive avoidance paradigms and morris water maze (MWM) were used as exteroceptive behavioral models to access learning and memory activity. Transfer latency, step down latency and escape latency parameters were evaluated plus maze, passive avoidance paradigm, morris water maze. Thereafter lipid peroxidation test, glutathione level and catalase activities were estimated in homogenized brain of mice.</p><p><strong>Results: </strong>Pretreatment of mice with EEPV (200mg/kg & 400mg/kg) significantly reduced scopolamine induced amnesia. The obtained data clearly revealed that there was increase in escape latency in MWM and also increase in step down latency in passive avoidance paradigm. Transfer latencey was found to be decrease in EPM and biochemical. Parameters were clearly satisfied the data as compared to negative control group which was indicative of cognitive improvement.</p><p><strong>Conclusion: </strong>P. vera fruit extract demonstrated to improve cognitive process by enhancing memory in different experimental paradigm such as EPM, passive avoidance and MWM when administered orally. 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引用次数: 5
摘要
目的:探讨黄连木果实对实验性记忆障碍小鼠的治疗作用。材料与方法:东莨菪碱(0.4mg mg/kg)致瑞士白化病小鼠记忆损伤。i.p)。将动物分为5组,每组6只,阳性对照组以羧甲基纤维素(CMC)为载药,阴性对照组以东莨菪碱为载药,标准组以多奈哌齐(5mg/kg i.p)加东莨菪碱。试验1组和试验2组分别以200mg/kg和400mg/kg P. o的剂量与东莨菪碱一起给药。采用抬高+迷宫(EPM)、被动回避范式和morris水迷宫(MWM)作为外感行为模型,研究受试者的学习记忆活动。再加上迷宫、被动回避范式、morris水迷宫,评估转移潜伏期、降压潜伏期和逃避潜伏期参数。随后进行小鼠脑匀浆脂质过氧化试验、谷胱甘肽水平及过氧化氢酶活性测定。结果:EEPV (200mg/kg和400mg/kg)预处理小鼠可显著减轻东莨菪碱所致的健忆症。结果表明,MWM模式下的逃避潜伏期增加,被动回避模式下的降压潜伏期增加。EPM和生化的传递潜伏期降低。与阴性对照组相比,参数明显满足,表明认知改善。结论:芦荟果提取物在EPM、被动回避和MWM等不同的实验范式下均能通过增强记忆来改善认知过程。因此,探索这种植物在治疗记忆障碍方面的潜力是值得的。
Pharmacological Approach of Pistacia Vera Fruit to Assess Learning and Memory Potential in Chemically-Induced Memory Impairment in Mice.
Objective: The present study was designed to investigate the potential of Pistacia vera (P. vera) fruits in experimental memory impairments in mice.
Material & methods: Memory impairment was induced in Swiss Albino mice by scopolamine (0.4mg mg/kg. i.p). Animals were divided into five separate groups of six animals each, positive control group received carboxy methyl cellulose (CMC) as vehicle, negative control group received scopolamine with vehicle, and standard group received donepezil (5mg/kg i.p) with Scopolamine. Ethanolic extract of P. vera (EEPV) at doses of 200mg/kg & 400mg/kg p.o were administered to group test1 & test 2 respectively along with scopolamine. Elevated plus maze (EPM), passive avoidance paradigms and morris water maze (MWM) were used as exteroceptive behavioral models to access learning and memory activity. Transfer latency, step down latency and escape latency parameters were evaluated plus maze, passive avoidance paradigm, morris water maze. Thereafter lipid peroxidation test, glutathione level and catalase activities were estimated in homogenized brain of mice.
Results: Pretreatment of mice with EEPV (200mg/kg & 400mg/kg) significantly reduced scopolamine induced amnesia. The obtained data clearly revealed that there was increase in escape latency in MWM and also increase in step down latency in passive avoidance paradigm. Transfer latencey was found to be decrease in EPM and biochemical. Parameters were clearly satisfied the data as compared to negative control group which was indicative of cognitive improvement.
Conclusion: P. vera fruit extract demonstrated to improve cognitive process by enhancing memory in different experimental paradigm such as EPM, passive avoidance and MWM when administered orally. Hence it would be worthwhile to explore the potential of this plant in the management of memory disorders.
期刊介绍:
Central Nervous System Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of new central nervous system agents. Containing a series of timely in-depth reviews written by leaders in the field covering a range of current topics, Central Nervous System Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in the field.