{"title":"舌螺虫α- n -乙酰半乳糖胺酶转化红细胞的研究。","authors":"Thomas J Malinski, Harkewal Singh","doi":"10.1155/2019/6972835","DOIUrl":null,"url":null,"abstract":"<p><p><i>Spirosoma linguale</i> is a free-living nonpathogenic organism. Like many other bacteria, <i>S. linguale</i> produces a cell-associated <i>α</i>-N-acetylgalactosaminidase. This work was undertaken to elucidate the nature of this activity. The recombinant enzyme was produced, purified, and examined for biochemical attributes. The purified enzyme was ~50 kDa active as a homodimer in solution. It catalyzed hydrolysis of <i>α</i>-N-acetylgalactosamine at pH 7. Calculated K<sub>M</sub> was 1.1 mM with k<sub>cat</sub> of 173 s<sup>-1</sup>. The described enzyme belongs to the GH109 family.</p>","PeriodicalId":11835,"journal":{"name":"Enzyme Research","volume":"2019 ","pages":"6972835"},"PeriodicalIF":0.0000,"publicationDate":"2019-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2019/6972835","citationCount":"2","resultStr":"{\"title\":\"Enzymatic Conversion of RBCs by <i>α</i>-N-Acetylgalactosaminidase from <i>Spirosoma linguale</i>.\",\"authors\":\"Thomas J Malinski, Harkewal Singh\",\"doi\":\"10.1155/2019/6972835\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Spirosoma linguale</i> is a free-living nonpathogenic organism. Like many other bacteria, <i>S. linguale</i> produces a cell-associated <i>α</i>-N-acetylgalactosaminidase. This work was undertaken to elucidate the nature of this activity. The recombinant enzyme was produced, purified, and examined for biochemical attributes. The purified enzyme was ~50 kDa active as a homodimer in solution. It catalyzed hydrolysis of <i>α</i>-N-acetylgalactosamine at pH 7. Calculated K<sub>M</sub> was 1.1 mM with k<sub>cat</sub> of 173 s<sup>-1</sup>. The described enzyme belongs to the GH109 family.</p>\",\"PeriodicalId\":11835,\"journal\":{\"name\":\"Enzyme Research\",\"volume\":\"2019 \",\"pages\":\"6972835\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-05-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1155/2019/6972835\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Enzyme Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/2019/6972835\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2019/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Enzyme Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2019/6972835","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2019/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 2
摘要
舌螺虫是一种自由生活的非致病性生物。像许多其他细菌一样，S. linguale产生与细胞相关的α- n -乙酰半乳糖胺酶。进行这项工作是为了阐明这项活动的性质。重组酶的产生、纯化和生化特性检测。纯化后的酶在溶液中为同二聚体，活性约50 kDa。在pH为7时催化α- n -乙酰半乳糖胺水解。计算KM为1.1 mM, kcat为173 s-1。所描述的酶属于GH109家族。
Enzymatic Conversion of RBCs by α-N-Acetylgalactosaminidase from Spirosoma linguale.
Spirosoma linguale is a free-living nonpathogenic organism. Like many other bacteria, S. linguale produces a cell-associated α-N-acetylgalactosaminidase. This work was undertaken to elucidate the nature of this activity. The recombinant enzyme was produced, purified, and examined for biochemical attributes. The purified enzyme was ~50 kDa active as a homodimer in solution. It catalyzed hydrolysis of α-N-acetylgalactosamine at pH 7. Calculated KM was 1.1 mM with kcat of 173 s-1. The described enzyme belongs to the GH109 family.
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