体积受限组织样本的跨平台代谢组学工作流程:应用于多囊肾病动物模型†

IF 3.743 Q2 Biochemistry, Genetics and Molecular Biology Molecular BioSystems Pub Date : 2017-08-02 DOI:10.1039/C7MB00245A
E. Sánchez-López, H. Happé, E. Steenvoorden, A. L. Crego, M. L. Marina, D. J. M. Peters and O. A. Mayboroda
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引用次数: 0

摘要

代谢谱分析提供了一个客观的观点,一个有机体的生理状态作为一个“功能”的代谢组成的测量样品。在这里,我们提出了一种简单的基于LC-MS的工作流程,用于体积受限样品的代谢分析,即小鼠肾脏的单个20 μm厚的组织学切片。该工作流程的主要思想是在hplc - ms运行后重复使用材料,即使用进样后小瓶中剩余的体积,然后引入相变步骤以进行HILIC-MS分析。为了测试工作流程的适用性及其提取有价值生物信息的能力,我们将其应用于多囊肾病(PKD)的动物模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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A cross-platform metabolomics workflow for volume-restricted tissue samples: application to an animal model for polycystic kidney disease†

Metabolic profiling provides an unbiased view of the physiological status of an organism as a “function” of the metabolic composition of a measured sample. Here, we propose a simple LC-MS based workflow for metabolic profiling of volume-restricted samples, namely individual 20 μm-thick histological sections of a mouse kidney. The main idea of this workflow is to re-use the material after an RPLC-MS run, namely using the volume remaining in the vial after injection, and then introducing a phase changing step to enable HILIC-MS analysis. To test the applicability of the workflow and its ability to extract valuable biological information, we applied it to an animal model of polycystic kidney disease (PKD).

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来源期刊
Molecular BioSystems
Molecular BioSystems 生物-生化与分子生物学
CiteScore
2.94
自引率
0.00%
发文量
0
审稿时长
2.6 months
期刊介绍: Molecular Omics publishes molecular level experimental and bioinformatics research in the -omics sciences, including genomics, proteomics, transcriptomics and metabolomics. We will also welcome multidisciplinary papers presenting studies combining different types of omics, or the interface of omics and other fields such as systems biology or chemical biology.
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