Asmahan A El Ezzi, Jordan M Clawson, Mohammed A El-Saidi, Wissam R Zaidan, Abigail Kovash, Jeremy Orellana, AnnaKarina Thornock, Ruhul H Kuddus
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The odds ratios (OR), 95% confidence intervals (CI), and <i>p</i> values of the allele frequencies and genotype ratios were calculated for establishing possible association of the alleles and/or genotypes and PCa and/or BPH.</p><p><strong>Results: </strong>The proportions of II, ID, and DD genotypes were significantly different from Hardy-Weinberg equilibrium for BPH and PCa groups (but not the control group), mostly due to overabundance of the ID genotypes. There was no significant difference in the I and D allele frequencies between the control groups and the affected groups. The ratio of (DD + ID)/II is significantly lower among the control group compared to the BPH group (RR = 8.92, <i>p</i> values of the allele frequencies and genotype ratios were calculated for establishing possible association of the alleles and/or genotypes and PCa and/or BPH. <i>p</i> values of the allele frequencies and genotype ratios were calculated for establishing possible association of the alleles and/or genotypes and PCa and/or BPH.</p><p><strong>Conclusions: </strong>Our data indicate that the D allele of the I/D polymorphisms of the ACE gene is associated with increased risk of BPH, and the ID genotype is a risk factor for both BPH and PCa among Lebanese males.</p>","PeriodicalId":20907,"journal":{"name":"Prostate Cancer","volume":"2020 ","pages":"5959134"},"PeriodicalIF":2.3000,"publicationDate":"2020-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7029258/pdf/","citationCount":"0","resultStr":"{\"title\":\"Association of Angiotensin I Converting Enzyme Insertion/287 bp Deletion Polymorphisms and Proliferative Prostatic Diseases among Lebanese Men.\",\"authors\":\"Asmahan A El Ezzi, Jordan M Clawson, Mohammed A El-Saidi, Wissam R Zaidan, Abigail Kovash, Jeremy Orellana, AnnaKarina Thornock, Ruhul H Kuddus\",\"doi\":\"10.1155/2020/5959134\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Angiotensin I converting enzyme (ACE) insertion (I) and 287 bp Alu repeat DNA fragment deletion (D) polymorphisms have been indicated in various cancers. Here, we investigated I/D polymorphisms in prostate cancer (PCa) and benign prostate hyperplasia (BPH) among Lebanese men.</p><p><strong>Methods: </strong>Blood DNA extracted from 69 control subjects, 69 subjects with clinically confirmed PCa, and 69 subjects with clinical BPH, all the subjects were aged 50 years or older, was subjected to the polymerase chain reaction. The PCR products were resolved in polyacrylamide gels to determine II, ID, and DD genotypes. The odds ratios (OR), 95% confidence intervals (CI), and <i>p</i> values of the allele frequencies and genotype ratios were calculated for establishing possible association of the alleles and/or genotypes and PCa and/or BPH.</p><p><strong>Results: </strong>The proportions of II, ID, and DD genotypes were significantly different from Hardy-Weinberg equilibrium for BPH and PCa groups (but not the control group), mostly due to overabundance of the ID genotypes. 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引用次数: 0
摘要
背景:血管紧张素 I 转换酶(ACE)插入(I)和 287 bp Alu 重复 DNA 片段缺失(D)多态性已被证实与多种癌症有关。在此,我们对黎巴嫩男性前列腺癌(PCa)和良性前列腺增生(BPH)中的 I/D 多态性进行了调查:方法:对从 69 名对照组受试者、69 名临床确诊 PCa 受试者和 69 名临床良性前列腺增生症受试者(所有受试者年龄均在 50 岁或以上)身上提取的血液 DNA 进行聚合酶链反应。聚合酶链反应产物在聚丙烯酰胺凝胶中解析,以确定 II、ID 和 DD 基因型。计算等位基因频率和基因型比率的几率比(OR)、95% 置信区间(CI)和 p 值,以确定等位基因和/或基因型与 PCa 和/或良性前列腺增生症的可能关联:在良性前列腺增生症组和 PCa 组(而非对照组)中,II、ID 和 DD 基因型的比例与 Hardy-Weinberg 平衡有显著差异,这主要是由于 ID 基因型过多所致。对照组和患病组的 I 和 D 等位基因频率没有明显差异。与良性前列腺增生组相比,对照组的(DD + ID)/II 比率明显较低(RR = 8.92,计算等位基因频率和基因型比率的 p 值,以确定等位基因和/或基因型与 PCa 和/或良性前列腺增生可能存在的关联):我们的数据表明,ACE 基因 I/D 多态性的 D 等位基因与良性前列腺增生症风险增加有关,而 ID 基因型是黎巴嫩男性良性前列腺增生症和 PCa 的风险因素。
Association of Angiotensin I Converting Enzyme Insertion/287 bp Deletion Polymorphisms and Proliferative Prostatic Diseases among Lebanese Men.
Background: Angiotensin I converting enzyme (ACE) insertion (I) and 287 bp Alu repeat DNA fragment deletion (D) polymorphisms have been indicated in various cancers. Here, we investigated I/D polymorphisms in prostate cancer (PCa) and benign prostate hyperplasia (BPH) among Lebanese men.
Methods: Blood DNA extracted from 69 control subjects, 69 subjects with clinically confirmed PCa, and 69 subjects with clinical BPH, all the subjects were aged 50 years or older, was subjected to the polymerase chain reaction. The PCR products were resolved in polyacrylamide gels to determine II, ID, and DD genotypes. The odds ratios (OR), 95% confidence intervals (CI), and p values of the allele frequencies and genotype ratios were calculated for establishing possible association of the alleles and/or genotypes and PCa and/or BPH.
Results: The proportions of II, ID, and DD genotypes were significantly different from Hardy-Weinberg equilibrium for BPH and PCa groups (but not the control group), mostly due to overabundance of the ID genotypes. There was no significant difference in the I and D allele frequencies between the control groups and the affected groups. The ratio of (DD + ID)/II is significantly lower among the control group compared to the BPH group (RR = 8.92, p values of the allele frequencies and genotype ratios were calculated for establishing possible association of the alleles and/or genotypes and PCa and/or BPH. p values of the allele frequencies and genotype ratios were calculated for establishing possible association of the alleles and/or genotypes and PCa and/or BPH.
Conclusions: Our data indicate that the D allele of the I/D polymorphisms of the ACE gene is associated with increased risk of BPH, and the ID genotype is a risk factor for both BPH and PCa among Lebanese males.
期刊介绍:
Prostate Cancer is a peer-reviewed, Open Access journal that provides a multidisciplinary platform for scientists, surgeons, oncologists and clinicians working on prostate cancer. The journal publishes original research articles, review articles, and clinical studies related to the diagnosis, surgery, radiotherapy, drug discovery and medical management of the disease.