{"title":"神经元多糖体复合体:神经末梢的分泌机制","authors":"Mzia G Zhvania, Nino Pochkidze","doi":"10.15190/d.2017.7","DOIUrl":null,"url":null,"abstract":"<p><p>Neuronal porosomes are 15 nm cup-shaped lipoprotein secretory machines composed of nearly 30 proteins present at the presynaptic membrane, that have been investigated using multiple imaging modalities, such as electron microscopy, atomic force microscopy, and solution X-ray. Synaptic vesicles transiently dock and fuse at the base of the porosome cup facing the cytosol, by establishing a fusion pore for neurotransmitter release. Studies on the morphology, dynamics, isolation, composition, and reconstitution of the neuronal porosome complex provide a molecular understanding of its structure and function. In the past twenty years, a large body of evidence has accumulated on the involvement of the neuronal porosome proteins in neurotransmission and various neurological disorders. In light of these findings, this review briefly summarizes our current understanding of the neuronal porosome complex, the secretory nanomachine at the nerve terminal.</p>","PeriodicalId":72829,"journal":{"name":"Discoveries (Craiova, Romania)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941571/pdf/","citationCount":"0","resultStr":"{\"title\":\"Neuronal Porosome Complex: Secretory Machinery at the Nerve Terminal.\",\"authors\":\"Mzia G Zhvania, Nino Pochkidze\",\"doi\":\"10.15190/d.2017.7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Neuronal porosomes are 15 nm cup-shaped lipoprotein secretory machines composed of nearly 30 proteins present at the presynaptic membrane, that have been investigated using multiple imaging modalities, such as electron microscopy, atomic force microscopy, and solution X-ray. Synaptic vesicles transiently dock and fuse at the base of the porosome cup facing the cytosol, by establishing a fusion pore for neurotransmitter release. Studies on the morphology, dynamics, isolation, composition, and reconstitution of the neuronal porosome complex provide a molecular understanding of its structure and function. In the past twenty years, a large body of evidence has accumulated on the involvement of the neuronal porosome proteins in neurotransmission and various neurological disorders. In light of these findings, this review briefly summarizes our current understanding of the neuronal porosome complex, the secretory nanomachine at the nerve terminal.</p>\",\"PeriodicalId\":72829,\"journal\":{\"name\":\"Discoveries (Craiova, Romania)\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-07-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941571/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Discoveries (Craiova, Romania)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15190/d.2017.7\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Discoveries (Craiova, Romania)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15190/d.2017.7","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
神经元孔体是一种 15 纳米的杯状脂蛋白分泌机器,由突触前膜上的近 30 种蛋白质组成,研究人员利用电子显微镜、原子力显微镜和溶液 X 射线等多种成像模式对其进行了研究。突触小泡在面向细胞质的孔杯底部瞬时对接和融合,通过建立融合孔释放神经递质。通过对神经元孔泡复合体的形态、动力学、分离、组成和重组的研究,人们从分子角度了解了它的结构和功能。在过去的二十年中,已经积累了大量关于神经元孔体蛋白参与神经传递和各种神经系统疾病的证据。鉴于这些发现,本综述简要总结了我们目前对神经元孔体复合体--神经末梢的分泌型纳米机器--的理解。
Neuronal Porosome Complex: Secretory Machinery at the Nerve Terminal.
Neuronal porosomes are 15 nm cup-shaped lipoprotein secretory machines composed of nearly 30 proteins present at the presynaptic membrane, that have been investigated using multiple imaging modalities, such as electron microscopy, atomic force microscopy, and solution X-ray. Synaptic vesicles transiently dock and fuse at the base of the porosome cup facing the cytosol, by establishing a fusion pore for neurotransmitter release. Studies on the morphology, dynamics, isolation, composition, and reconstitution of the neuronal porosome complex provide a molecular understanding of its structure and function. In the past twenty years, a large body of evidence has accumulated on the involvement of the neuronal porosome proteins in neurotransmission and various neurological disorders. In light of these findings, this review briefly summarizes our current understanding of the neuronal porosome complex, the secretory nanomachine at the nerve terminal.