应用人淋巴样细胞评价抗病毒药物对人腺病毒19型的作用:与西多福韦相比,Zalcitabine具有更强的活性。

Q2 Pharmacology, Toxicology and Pharmaceutics Antiviral Chemistry and Chemotherapy Pub Date : 2020-01-01 DOI:10.1177/2040206620921319
Kohsuke Nakagawara, Hironori Hayashi, Kumi Kawaji, Mina Sasano, Eiichi N Kodama
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引用次数: 2

摘要

人腺病毒19型(HAdV-19)是引起角膜结膜炎流行的主要原因。角膜结膜炎的爆发对人类健康是一个问题,特别是对婴儿、老年人和免疫功能低下的个体。然而,抗hav药物的开发一直受到不方便的筛选系统的阻碍;因此,开发一种简单的筛选方法是非常必要的。在这项研究中,我们发现HAdV-19可以感染由人t细胞白血病病毒(MT-2细胞)转化的人淋巴样细胞系。MT-2细胞支持HAdV-19复制,并在感染后5天内表现出明显的细胞病变效应。使用噻唑蓝溴化四唑(MTT)为基础的MT-2细胞比色法,我们能够检测先前报道的核苷/tide化合物的抗hadv -19活性,包括(S)-1-(3-羟基-2-膦基甲氧基丙基)胞嘧啶(西多福韦),2',3'-二脱氧胞苷(zalcitabine)和3'-脱氧-3'-氟胸苷(三氟啶)。与以往的方法相比,该系统是一种更简单、快速的抗hadv -19药物筛选方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Application of human lymphoid cells for the evaluation of antivirals against human adenovirus type 19: Zalcitabine has superior activity compared to cidofovir.

Human adenovirus type 19 (HAdV-19) is a major cause of the epidemic keratoconjunctivitis. Outbreaks of keratoconjunctivitis are problematic to human health, especially for infants, the elderly, and immunocompromised individuals. However, the development of anti-HAdV drugs has been hampered by inconvenient screening systems; therefore, development of a simple screening method is highly desirable. In this study, we identified that HAdV-19 can infect a human lymphoid cell line transformed with human T-cell leukemia virus (MT-2 cells). MT-2 cells supported HAdV-19 replication and showed apparent cytopathic effects within five days post-infection. Using a thiazolyl blue tetrazolium bromide (MTT)-based colorimetric assay on MT-2 cells, we were able to detect the anti-HAdV-19 activities of previously reported nucleoside/tide compounds, including (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (cidofovir), 2',3'-dideoxycytidine (zalcitabine) and 3'-deoxy-3'-fluorothymidine (trifluridine). Compared with previous methods, this system represents a more simple and rapid method to screen anti-HAdV-19 agents.

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来源期刊
Antiviral Chemistry and Chemotherapy
Antiviral Chemistry and Chemotherapy Pharmacology, Toxicology and Pharmaceutics-Pharmacology
CiteScore
5.20
自引率
0.00%
发文量
5
审稿时长
15 weeks
期刊介绍: Antiviral Chemistry & Chemotherapy publishes the results of original research concerned with the biochemistry, mode of action, chemistry, pharmacology and virology of antiviral compounds. Manuscripts dealing with molecular biology, animal models and vaccines are welcome. The journal also publishes reviews, pointers, short communications and correspondence.
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