线性阵列与SPF10 PCR-DEIA-LiPA25系统在男性HPV感染(HIM)研究中的应用

Deepti Bettampadi , Bradley A. Sirak , William J. Fulp , Martha Abrahamsen , Luisa L. Villa , Eduardo Lazcano-Ponce , Jorge Salmeron , Kimberly A. Isaacs-Soriano , Maria L. Baggio , Manuel Quiterio Trenado , Anna R. Giuliano
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引用次数: 4

摘要

在许多国家,由口腔人乳头瘤病毒(HPV)引起的口咽癌呈上升趋势。健康人的口腔HPV感染通常是通过使用口腔含漱液样本来检测的。然而,研究中口腔含漱液样本HPV基因分型的最佳方法尚未得到评估。材料与方法对1455例男性HPV感染(HIM)研究对象的口腔含漱液样本进行HPV基因分型,采用两种不同的方法:线性阵列法和SPF10 PCR-DEIA-LiPA25。评估两种检测方法对HPV个体型和HPV分组型、高危型、低危型、4-HPV疫苗分组型和9-HPV疫苗分组型的敏感性,以及两种检测方法之间的一致性。我们还研究了社会人口行为因素是否与两种分析之间的一致性有关。结果除HPV 70外,SPF10 PCR-DEIA-LiPA25检测口腔HPV的灵敏度均高于线性阵列。在不同HPV基因型(不包括HPV 70)中,SPF10 PCR-DEIA-LiPA25对线性阵列的检出率在1.0 ~ 9.0之间,在4价和9价HPV疫苗组中,检出率分别在3.8 ~ 4.4之间。社会人口行为因素与两种口腔HPV 16检测结果的不一致之间没有关联。讨论spf10 PCR-DEIA-LiPA25检测口腔含漱液中HPV的灵敏度高于线性阵列。鉴于口腔HPV感染检测在口腔HPV自然史研究和疫苗有效性评估中的重要性日益增加,我们建议使用SPF10 PCR-DEIA-LiPA25等灵敏度更高的方法检测口腔含漱液样本中的HPV。
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Oral HPV prevalence assessment by Linear Array vs. SPF10 PCR-DEIA-LiPA25 system in the HPV Infection in Men (HIM) study

Introduction

Oral human papillomavirus (HPV) attributable oropharyngeal cancers are on the rise in many countries. Oral HPV infections among healthy individuals are commonly detected using oral gargle samples. However, the optimal method for HPV genotyping oral gargle specimens in research studies has not been previously evaluated.

Materials and methods

Oral gargle samples from 1455 HPV Infection in Men (HIM) study participants were HPV genotyped using two different methods: Linear Array and the SPF10 PCR-DEIA-LiPA25. The sensitivity of the two tests for detecting individual HPV types and grouped HPV types, high-risk HPV, low-risk HPV, grouped 4-HPV-vaccine types, and grouped 9-HPV-vaccine-types, and the degree of concordance between the two tests was assessed. We also examined whether socio-demographic-behavioral factors were associated with concordance between the two assays.

Results

The sensitivity of SPF10 PCR-DEIA-LiPA25 was higher than Linear Array, with the exception of HPV 70, for the detection of oral HPV. The prevalence ratio of SPF10 PCR-DEIA-LiPA25 to Linear Array varied between 1.0 and 9.0 for individual HPV genotypes, excluding HPV 70, and between 3.8 and 4.4 for grouped 4-valent and 9-valent HPV vaccine types, respectively. There was no association between socio-demographic-behavioral factors and discordance in results between the two tests for oral HPV 16 detection.

Discussion

SPF10 PCR-DEIA-LiPA25 was more sensitive than Linear Array for detecting HPV in oral gargle samples. Given the growing importance of detecting oral HPV infection for research studies of oral HPV natural history and vaccine effectiveness evaluation, we recommend using methods with higher sensitivity such as SPF10 PCR-DEIA-LiPA25 for detecting HPV in oral gargle samples.

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来源期刊
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期刊介绍: The official Journal of the International Papillomavirus Society Papillomavirus Research (PVR), the Journal of HPV and other Small DNA Tumor Viruses publishes innovative papers related to all aspects of papillomaviruses and other small DNA tumor viruses. The official journal of the International Papillomavirus Society, PVR is an open access publication that aims to bring together virologists, immunologists, epidemiologists and clinicians working in the booming field of HPV and animal papillomaviruses, polyomaviruses and other small DNA tumor viruses and their associated diseases, in order to foster and facilitate interdisciplinary communication. The journal welcomes original research articles, reviews, short communications, opinion articles and regional update reports on papillomaviruses and other tumor viruses in the following sections: a. Biology of papillomaviruses and related viruses from life cycle to cancer b. Epidemiology etiology and natural history studies c. Natural and induced immunity including vaccine research d. Intervention studies and strategies including i. Clinical studies and trials ii. HPV treatments iii. HPV vaccination programs iv. Diagnostics and screening e. Infection and disease prevention, modeling studies f. Guidelines and public health recommendations g. HPV Studies in special populations Regional and local studies on these viruses.
期刊最新文献
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